Sex bias in utero alters ovarian reserve but not uterine capacity in female offspring†.
Sex bias in utero alters ovarian reserve but not uterine capacity in female offspring†.
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DOI：
10.1093/biolre/ioac208
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发表时间：
2023-02-13
影响因子：
3.6
通讯作者：
中科院分区：
文献类型：
--
作者：
胎儿所暴露的环境压力会影响出生后后代的一系列生理功能。我们的目的是确定在子宫内的类固醇激素对雌性后代的生殖潜力，使用猪模型的影响。在屠宰时（95-115 kg）收集雌性偏向（>65%雌性，n = 15）、非偏向（45-54.9%雌性，n = 15）和雄性偏向（<35%雌性，n = 9）窝猪的生殖道。处理卵巢和子宫角用于H&E或免疫组织化学。采用Levene检验分析组内数据的变异性，同时采用R.在卵巢储备中，出生体重与性别比存在显著的相互作用（P = 0.015），来自雄性偏向窝的低出生体重猪具有较高的原始卵泡数量，而来自雌性偏向窝的猪具有相反的趋势。性别差异对子宫内膜腺体发育无影响。较低的出生体重降低了子宫内膜中发现的腺体的比例（P = 0.045），并且在男性偏置和女性偏置的窝仔中变化更大（P = 0.026）。从男性偏向的窝原始卵泡的变异性大于非和女性偏向的窝（P = 0.014）。同样，子宫内膜间质细胞核有一个更大的范围内，男性和女性的偏见比非偏见窝（P = 0.028）。一个重要的发现是来自雄性偏置窝的雌性卵巢中的原始卵泡和来自雄性和雌性偏置窝的雌性子宫内膜中的基质细胞计数的变异性更大。这可能是膨胀的变异性繁殖成功的女性从男性偏见的窝。在极端性别比例下子宫内妊娠的女性，出生后卵巢储备功能发生改变，但子宫容量不变。
Environmental stressors to which a fetus is exposed affect a range of physiological functions in postnatal offspring. We aimed to determine the in utero effect of steroid hormones on the reproductive potential of female offspring using a porcine model. Reproductive tracts of pigs from female-biased (>65% female, n = 15), non-biased (45–54.9% female, n = 15), and male-biased litters (<35% females, n = 9) were collected at slaughter (95–115 kg). Ovaries and uterine horns were processed for H&E or immunohistochemistry. Variability of data within groups was analyzed with a Levene’s test, while data were analyzed using mixed linear models in R. In the ovarian reserve, there was a significant birth weight by sex ratio interaction (P = 0.015), with low birth weight pigs from male-biased litters having higher numbers of primordial follicles with opposite trends seen in pigs from female-biased litters. Sex bias held no effect on endometrial gland development. A lower birth weight decreased the proportion of glands found in the endometrium (P = 0.045) and was more variable in both male-biased and female-biased litters (P = 0.026). The variability of primordial follicles from male-biased litters was greater than non- and female-biased litters (P = 0.014). Similarly, endometrial stromal nuclei had a greater range in male- and female-biased litters than non-biased litters (P = 0.028). A crucial finding was the greater variability in primordial follicles in the ovaries from females derived from male-biased litters and stromal cell count in the endometrium of females from male- and female-biased litters. This could be inflating the variability of reproductive success seen in females from male-biased litters. Females gestated in utero in extremes of sex ratios have altered ovarian reserve postnatally but not uterine capacity.
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影响因子：
3.3
作者：
BAZER, FW
通讯作者：
BAZER, FW
DOI：
10.1530/jrf.0.1150097
发表时间：
1999-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Drickamer, LC;Rosenthal, TL;Arthur, RD
通讯作者：
Arthur, RD
影响因子：
4.1
作者：
Faddy, MJ
通讯作者：
Faddy, MJ
影响因子：
2
作者：
Fonseca, Carlos;Alves da Silva, Antonio;Soares, Amadeu M. V. M.
通讯作者：
Soares, Amadeu M. V. M.
影响因子：
3.6
作者：
Gray, CA;Taylor, KM;Spencer, TE
通讯作者：
Spencer, TE