Dietary Blueberry Ameliorates Vascular Complications in Diabetic Mice Possibly through NOX4 and Modulates Composition and Functional Diversity of Gut Microbes.
Dietary Blueberry Ameliorates Vascular Complications in Diabetic Mice Possibly through NOX4 and Modulates Composition and Functional Diversity of Gut Microbes.
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DOI：
10.1002/mnfr.202100784
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发表时间：
2022-04
影响因子：
5.2
通讯作者：
Babu, Pon Velayutham Anandh
中科院分区：
文献类型：
--
作者：
Petersen, Chrissa;Bharat, Divya;Wankhade, Umesh D.;Kim, Ji-Seok;Cutler, Brett Ronald;Denetso, Christopher;Gholami, Samira;Nelson, Samantha;Bigley, Jessica;Johnson, Aspen;Chintapalli, Sree, V;Piccolo, Brian D.;Babu, Adhini Kuppuswamy Satheesh;Paz, Henry A.;Shankar, Kartik;Symons, J. David;Babu, Pon Velayutham Anandh
关键词：
在糖尿病中，内皮炎症和功能障碍在血管疾病的发展中起着关键作用。我们研究了饮食蓝莓对糖尿病小鼠血管并发症和肠道微生物组的影响。7周龄糖尿病db/db小鼠食用标准饮食（db/db）或补充有3.8%冻干蓝莓的饮食（db/db+BB）10周。对照db/+小鼠喂食标准饮食（db/+）。通过测量单核细胞与脉管系统的结合和炎症标志物来评估血管炎症。采用等长张力法评估肠系膜动脉功能。与db/+小鼠相比，db/db小鼠表现出增强的血管炎症和降低的内皮依赖性血管舒张，但这些在db/db+BB小鼠中得到改善。与db/db小鼠相比，蓝莓补充降低了db/db+BB小鼠主动脉血管和血管内皮细胞（EC）中NOX 4和IκKβ的表达。蓝莓代谢物血清减少葡萄糖和棕榈酸诱导的小鼠主动脉内皮细胞的内皮炎症。此外，蓝莓补充剂增加了糖尿病小鼠的肠道微生物，并调节了肠道微生物的功能潜力。膳食蓝莓抑制血管炎症，减轻动脉内皮功能障碍，并支持糖尿病小鼠体内肠道微生物的生长。蓝莓的内皮特异性血管益处是通过NOX 4信号传导介导的。蓝莓的膳食补充剂抑制血管炎症，改善血管功能障碍，并增加糖尿病小鼠有益肠道微生物的丰度。蓝莓的循环代谢物似乎对血管内皮有好处。我们的研究提供了强有力的概念证据，考虑蓝莓补充剂作为一种辅助治疗，以减少糖尿病血管并发症。
In diabetes, endothelial inflammation and dysfunction plays a pivotal role in the development of vascular disease. We investigated the effect of dietary blueberries on vascular complications and gut microbiome in diabetic mice. Seven-week-old diabetic db/db mice consumed a standard diet (db/db) or a diet supplemented with 3.8% freeze-dried blueberry (db/db+BB) for 10 weeks. Control db/+ mice fed a standard diet (db/+). Vascular inflammation was assessed by measuring the monocyte binding to vasculature and inflammatory markers. Isometric tension procedures were used to assess mesenteric artery function. db/db mice exhibited enhanced vascular inflammation and reduced endothelial-dependent vasorelaxation as compared to db/+ mice but these were improved in db/db+BB mice. Blueberry supplementation reduced the expression of NOX4 and IκKβ in the aortic vessel and vascular endothelial cells (ECs) isolated from db/db+BB compared to db/db mice. The blueberry metabolites serum reduced glucose and palmitate induced endothelial inflammation in mouse aortic ECs. Further, blueberry supplementation increases commensal microbes and modulates the functional potential of gut microbes in diabetic mice. Dietary blueberry suppresses vascular inflammation, attenuates arterial endothelial dysfunction and supports the growth of commensal microbes in diabetic mice. The endothelial specific vascular benefits of blueberries are mediated through NOX4 signaling. Dietary supplementation of blueberry suppresses vascular inflammation, improves vascular dysfunction and increases the abundance of beneficial gut microbes in diabetic mice. The circulating metabolites of blueberry appears to exert benefits on the vascular endothelium. Our study provides strong proof of concept to consider blueberry supplementation as an adjunct therapy to lessen vascular complications in diabetes.
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影响因子：
6.1
作者：
Hidalgo, Maria;Oruna-Concha, M. Jose;de Pascual-Teresa, Sonia
通讯作者：
de Pascual-Teresa, Sonia
影响因子：
3.7
作者：
Dardmeh F;Alipour H;Gazerani P;van der Horst G;Brandsborg E;Nielsen HI
通讯作者：
Nielsen HI
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
5.2
作者：
de Ferrars, Rachel M.;Cassidy, Aedin;Kay, Colin D.
通讯作者：
Kay, Colin D.
影响因子：
7.3
作者：
de Ferrars RM;Czank C;Zhang Q;Botting NP;Kroon PA;Cassidy A;Kay CD
通讯作者：
Kay CD