Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model.
Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model.
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DOI：
10.1371/journal.pone.0185964
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nielsen HI
中科院分区：
文献类型：
--
作者：
Dardmeh F;Alipour H;Gazerani P;van der Horst G;Brandsborg E;Nielsen HI
益生菌已被建议在几种医疗条件下作为药理产品的替代品，包括调节肥胖，这经常与较差的精液质量有关。然而，益生菌对男性生育能力的影响研究较少。本研究评估了鼠李糖乳杆菌PB01(DSM-14870)对正常体重(NW)和饮食诱导肥胖(DIO)模型精子运动参数的影响。将NW和DIO C57BL/6NTac小鼠随机分为两个亚组，每天给予或不给予鼠李糖单剂量(1x109CFU)，连续四周。使用精子分类分析系统评估精子的运动学和运动学，以及血脂和生殖激素水平。添加益生菌后，NW组和DIO组的血清睾酮、黄体生成素和卵泡刺激素水平均显著升高(P<；0.05)，精子运动速度(VSL、VCL和VAP)显著增加(P<；0.05)，运动精子百分率和静止精子百分率显著降低。添加益生菌的DIO组和NW组的其他运动参数(LIN、STR、ALH和BCF)也在10%的显著水平上增加。添加益生菌的DIO小鼠与DIO对照组相比，显示出显著更高的渐进性精子运动百分比。本研究证明了鼠李糖PB01作为一种调节剂的潜力，对体重减轻和生殖激素有积极的作用，显著改善男性DIO模型的精子活力和运动参数。
Probiotics have been proposed as alternatives to pharmacological products in several medical conditions including the modulation of obesity, which is frequently associated with poor semen quality. However, effects of probiotics on male fertility have been less investigated. This study assessed the effect of Lactobacillus rhamnosus PB01 (DSM-14870) on sperm kinematic parameters in Normal-weight (NW) and diet-induced obese (DIO) models. NW and DIO C57BL/6NTac mice were divided into two subgroups with or without a single daily dose (1x109CFU) of L. rhamnosus for four weeks. Sperm motility and kinematics together with blood lipid profiles and reproductive hormone levels were assessed using the sperm class analyzer system. Probiotic supplementation increased serum testosterone, LH and FSH levels in both NW and DIO groups resulting in significantly (P<0.05) higher velocity (VSL, VCL and VAP) and percentages of progressively motile sperm and significantly lower percentages of immotile sperm. Other kinematic parameters (Lin, STR, ALH and BCF) were also increased in both probiotic supplemented DIO and NW groups at the 10% level of significance. Probiotic supplemented DIO mice demonstrated significantly higher percentages of progressively motile sperm versus DIO controls. This study demonstrated the potential of L. rhamnosus PB01 as a regulatory agent with positive effects on weight loss and reproductive-hormones, significantly improving sperm motility and kinematic parameters in male DIO models.
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