Transcriptome and QTL analyses reveal candidate genes for fiber quality in Upland cotton
Transcriptome and QTL analyses reveal candidate genes for fiber quality in Upland cotton
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转录组和 QTL 分析揭示陆地棉纤维品质的候选基因
DOI：
10.1016/j.cj.2019.05.002
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Zhongxu Lin
中科院分区：
文献类型：
--
作者：
Hantao Wang;Ruiting Zhang;Chao Shen;Ximei Li;De Zhu;Zhongxu Lin
随着人们对高品质棉花需求的增加，寻找与棉花纤维发育相关的基因进行分子改良是十分必要的。在这项研究中，780个差异表达基因(DEG)在棉花花后10 天的纤维发育过程中被鉴定出来。DH962和G.赫苏图姆。济棉5号利用RNA-seq.在同一亲本的15个稳定QTL中，分别有4个、3个、6个、1个和1个QTL分别与纤维长度(FL)、纤维强力(FS)、麦克隆值(MIC)、纤维伸长(FE)和纤维长度整齐度(Fu)相关。Gh_A01G01G0453(GhDTX19)、Gh_D07G1581和Gh_D04G0942在5和10个DPA纤维中特异表达，Gh_D04G0942(Gh_D04G0942)在除雌蕊外的其他组织中低表达，Gh_D07G1799(GhGAUT9)、Gh_D11G0326(GhV29)、Gh_D11G0333(GhTCP14)和Gh_D11G0334(GhNRP2)在5和10个DPA纤维中特异表达，Gh_A01G0397(GhGAUT9)、Gh_D11G0326(GhV29)、Gh_D11G0333(GhTCP14)和Gh_D11G0334(GhNRP2)在5和10个DPA纤维中特异表达，Gh_D01G0397(GhGAUT9)、Gh_D11G0326(GhV29)、Gh_D11G0333(GhTCP14)和Gh_D11G0334(GhNRP2)在5和10个DPA纤维中特异表达，Gh_D01G0397(GhGAUT9)、Gh_D11G0326(GhV29)、Gh_D11G0333(GhTCP14)和Gh_D11G0334(GhNRP2)在5和10个DPA纤维中特异表达，Gh_A01G0397(GhGAUT9)、Gh_D11G0326(GhV29)、Gh_D11G0333(GhTCP14)和Gh_D11G0334(GhNRP2)在5和10个DPA纤维中特异表达这些结果提示了棉花纤维品质分子改良的候选基因。
With increasing demand for high-quality cotton, it is desirable to identify genes involved in fiber development for molecular improvement of cotton. In this study, 780 differentially expressed genes (DEGs) were identified in developing fibers at 10 days post-anthesis (DPA) inGossypium hirsutumacc. DH962 andG. hirsutumcv. Jimian 5 using RNA-seq. Of 15 stable QTL for fiber quality identified in the same two parents in previous studies, 4, 3, 6, 1, and 1 QTL were associated with fiber length (FL), fiber strength (FS), micronaire (MIC), fiber elongation (FE) and fiber length uniformity ratio (FU), respectively. Integration of DEGs and QTL allowed the identification of 31 genes in 9 QTL regions, of which 25 were highly expressed in fibers based on the transcriptome datasets and 9 were preferentially expressed in different stages of fiber development.Gh_A01G0453(GhDTX19),Gh_D07G1581andGh_D04G0942were expressed specifically in 5 and 10 DPA fibers, withGh_D04G0942showing low expression in other tissues except pistil.Gh_D07G1799(GhGAUT9),Gh_D11G0326(GhVPS29),Gh_D11G0333(GhTCP14), andGh_D11G0334(GhNRP2) were preferentially expressed in 5 or 10 DPA fibers;Gh_A01G0397(GhABCG10) andGh_D07G1744were expressed specifically in 20 and 25 DPA fibers. These results suggest candidate genes for molecular improvement of cotton fiber quality.
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影响因子：
3.7
作者：
Qin LX;Rao Y;Li L;Huang JF;Xu WL;Li XB
通讯作者：
Li XB
影响因子：
4.4
作者：
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影响因子：
14.9
作者：
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通讯作者：
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DOI：
10.1105/tpc.106.047399
发表时间：
2006-11
期刊：
The Plant Cell Online
影响因子：
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作者：
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通讯作者：
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影响因子：
4.4
作者：
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