Cotton GalT1 encoding a putative glycosyltransferase is involved in regulation of cell wall pectin biosynthesis during plant development.
Cotton GalT1 encoding a putative glycosyltransferase is involved in regulation of cell wall pectin biosynthesis during plant development.
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DOI：
10.1371/journal.pone.0059115
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li XB
中科院分区：
文献类型：
--
作者：
Qin LX;Rao Y;Li L;Huang JF;Xu WL;Li XB
阿拉伯半乳聚糖蛋白（AGPs）是一组高度糖基化的蛋白质，其在整个植物界中被发现。迄今为止，使AGP骨架糖基化的糖基转移酶仍在很大程度上未知。在这项研究中，基因（GhGalT 1）编码一个假定的β-1，3-半乳糖基转移酶（GalT）的棉花。GhGalT 1属于CAZy GT31家族，是一种II型膜蛋白，其N端含有一个跨膜结构域，C端含有一个半乳糖基转移酶功能域。亚细胞定位实验表明GhGalT 1定位于高尔基体。RT-PCR分析表明GhGalT 1在下胚轴、根、纤维和胚珠中表达量较高。GhGalT 1在拟南芥中的过表达促进了植物的生长和代谢。结果表明，转基因植株的初生根长，叶绿素含量高，光合效率高，生物量增加，对外源D-阿拉伯糖和D-半乳糖的耐受性增强。此外，细胞壁组分的单糖组成的气相色谱分析（GC）表明，果胶在转基因植物中发生了变化，与野生型相比。3个参与果胶生物合成的基因（GAUT 8、GAUT 9和xgd 1）在转基因株系中显著上调。这些数据表明，GhGalT 1可能参与调节植物发育所需的果胶生物合成。
Arabinogalactan proteins (AGPs), are a group of highly glycosylated proteins that are found throughout the plant kingdom. To date, glycosyltransferases that glycosylate AGP backbone have remained largely unknown. In this study, a gene (GhGalT1) encoding a putative β-1,3-galactosyltransferase (GalT) was identified in cotton. GhGalT1, belonging to CAZy GT31 family, is the type II membrane protein that contains an N-terminal transmembrane domain and a C-terminal galactosyltransferase functional domain. A subcellular localization assay demonstrated that GhGalT1 was localized in the Golgi apparatus. RT-PCR analysis revealed that GhGalT1 was expressed at relatively high levels in hypocotyls, roots, fibers and ovules. Overexpression of GhGalT1 in Arabidopsis promoted plant growth and metabolism. The transgenic seedlings had much longer primary roots, higher chlorophyll content, higher photosynthetic efficiency, the increased biomass, and the enhanced tolerance to exogenous D-arabinose and D-galactose. In addition, gas chromatography (GC) analysis of monosaccharide composition of cell wall fractions showed that pectin was changed in the transgenic plants, compared with that of wild type. Three genes (GAUT8, GAUT9 and xgd1) involved in pectin biosynthesis were dramatically up-regulated in the transgenic lines. These data suggested that GhGalT1 may be involved in regulation of pectin biosynthesis required for plant development.
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影响因子：
11.6
作者：
Rosti, Johannes;Barton, Christopher J.;Seifert, Georg J.
通讯作者：
Seifert, Georg J.
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7.4
作者：
Gaspar, YM;Nam, J;Bacic, A
通讯作者：
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