Campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes.
Campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes.
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DOI：
10.1371/journal.ppat.0040014
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发表时间：
2008-01
期刊：
影响因子：
6.7
通讯作者：
Galán JE
中科院分区：
文献类型：
--
作者：
Watson RO;Galán JE
空肠弯曲菌是世界范围内感染性腹泻的主要病原之一，但其致病机制尚不清楚。这种细菌可以进入肠上皮细胞，这被认为是其持续感染和引起疾病的能力的重要因素。我们发现C.空肠能够在肠上皮细胞内存活。然而，细胞内细菌的回收需要在限氧条件下预培养，这表明C。空肠在细胞内环境中经历显著的生理变化。我们还发现，在上皮细胞中，C。含有空泡的空泡在独特的小窝依赖性进入途径之后立即偏离典型的内吞途径，从而避免递送到溶酶体中。相反，在巨噬细胞中，C.空肠被递送到溶酶体，并因此被迅速杀死。综合起来，这些研究表明，C。空肠已经进化出在宿主细胞内生存的特殊适应。 空肠弯曲菌是美国食源性疾病的最常见原因之一，也是世界各地腹泻病的主要原因。通过口腔途径感染后，这种细菌侵入肠上皮细胞，这是一种对其致病能力很重要的特性。通常，进入细胞的细菌和其他物质会移动到称为溶酶体的隔间，在那里酸性混合酶将其分解。本研究表明，C.空肠可以通过避免递送到溶酶体而在肠上皮细胞内存活。相反，在巨噬细胞中，这是一种具有吞噬和杀死细菌能力的特化细胞，C。空肠无法避免输送到溶酶体中，因此迅速被杀死。这些研究有助于解释C.空肠。
Campylobacter jejuni is one of the major causes of infectious diarrhea world-wide, although relatively little is know about its mechanisms of pathogenicity. This bacterium can gain entry into intestinal epithelial cells, which is thought to be important for its ability to persistently infect and cause disease. We found that C. jejuni is able to survive within intestinal epithelial cells. However, recovery of intracellular bacteria required pre-culturing under oxygen-limiting conditions, suggesting that C. jejuni undergoes significant physiological changes within the intracellular environment. We also found that in epithelial cells the C. jejuni–containing vacuole deviates from the canonical endocytic pathway immediately after a unique caveolae-dependent entry pathway, thus avoiding delivery into lysosomes. In contrast, in macrophages, C. jejuni is delivered to lysosomes and consequently is rapidly killed. Taken together, these studies indicate that C. jejuni has evolved specific adaptations to survive within host cells. Campylobacter jejuni is one of the most common causes of food-borne illness in the United States and a major cause of diarrheal disease throughout the world. After infection through the oral route, this bacterium invades the cells of the intestinal epithelium, a property that is important for its ability to cause disease. Usually, bacteria and other material entering the cell move to compartments called lysosomes, where an acidic mix of enzymes breaks it down. This study shows that C. jejuni can survive within intestinal epithelial cells by avoiding delivery to lysosomes. In contrast, in macrophages, which are specialized cells with the capacity to engulf and kill bacteria, C. jejuni cannot avoid delivery into lysosomes and consequently is rapidly killed. These studies help explain an important virulence attribute of C. jejuni.
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突变动力蛋白的诱导特异性阻断内吞涂层囊泡的形成。
影响因子：
7.8
作者：
Damke, H;Baba, T;Warnock, D E;Schmid, S L
通讯作者：
Schmid, S L
DOI：
10.1083/jcb.137.1.113
发表时间：
1997-04-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blocker A;Severin FF;Burkhardt JK;Bingham JB;Yu H;Olivo JC;Schroer TA;Hyman AA;Griffiths G
通讯作者：
Griffiths G
DOI：
10.1083/jcb.131.6.1435
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Feng Y;Press B;Wandinger-Ness A
通讯作者：
Wandinger-Ness A
影响因子：
3.6
作者：
Hendrixson, DR;DiRita, VJ
通讯作者：
DiRita, VJ
影响因子：
3.1
作者：
FAUCHERE, JL;ROSENAU, A;PFISTER, A
通讯作者：
PFISTER, A