UV light assisted antibiotics for eradication of in vitro biofilms.
UV light assisted antibiotics for eradication of in vitro biofilms.
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DOI：
10.1038/s41598-018-34340-8
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发表时间：
2018-11-05
影响因子：
4.6
通讯作者：
Petersen PM
中科院分区：
文献类型：
--
作者：
Argyraki A;Markvart M;Stavnsbjerg C;Kragh KN;Ou Y;Bjørndal L;Bjarnsholt T;Petersen PM
抗生素的过度使用正在加速细菌的耐药性，因此需要减少治疗中抗生素的用量。在这里，我们在体外证明，296纳米左右的窄范围内的特定波长能够比抗生素更有效地消灭生物膜状态（生长24小时）的细菌，并且照射和抗生素的结合甚至更好，引入了光辅助抗生素的新概念。研究的波长范围为 249 nm 至 338 nm，步长约为 5 nm。这种新颖的概念包括紫外线照射治疗和妥布霉素治疗，可以显着减少根除成熟细菌生物膜所需的抗生素用量。将所提出的光辅助抗生素方法的效率与组合抗生素治疗和高浓度抗生素单一疗法进行比较。光辅助抗生素和浓度高约 10 倍的抗生素单一疗法对成熟生物膜的根除效果是相当的。目前的成就可能会推动光辅助抗生素治疗感染的发展。
The overuse of antibiotics is accelerating the bacterial resistance, and therefore there is a need to reduce the amount of antibiotics used for treatment. Here, we demonstrate in vitro that specific wavelengths in a narrow range around 296 nm are able to eradicate bacteria in the biofilm state (grown for 24 hours) more effectively, than antibiotics and the combination of irradiation and antibiotics is even better, introducing a novel concept light assisted antibiotics. The investigated wavelength range was 249 nm to 338 nm with an approximate step of 5 nm. The novel concept that consists of a UV irradiation treatment followed by a tobramycin treatment can significantly reduce the amount of antibiotics needed for eradicating mature bacterial biofilms. The efficiency of the proposed light assisted antibiotics method was compared to combinatory antibiotic treatment and highly concentrated antibiotic monotherapy. The eradication efficacies, on mature biofilms, achieved by light assisted antibiotic and by the antibiotic monotherapy at approximately 10-fold higher concentration, were equivalent. The present achievement could motivate the development of light assisted antibiotic treatments for treating infections.
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