MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens.
MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens.
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DOI：
10.1186/s13071-023-05957-0
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发表时间：
2023-09-13
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
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作者：
雌性蚊子交配后需要一顿血来发育它们的卵，这种行为会导致病原体的传播。因此，了解雌性蚊子繁殖的分子调控对于控制蚊媒种群至关重要。在本研究中，我们报道了针对5-HTR1(编码分泌的5-羟色胺受体1)的microRNA-989(miR-989)是蚊子繁殖所必需的。用实时定量逆转录聚合酶链式反应(RT-qPCR)检测miR-989的时空表达谱。设计并合成了miR-989反义寡核苷酸和反义寡核苷酸阴性对照(NC)，以下调雌性蚊体内源性miR-989的表达。RNA测序分析miR-989缺失对卵巢的影响。用RNA杂交法和双荧光素酶试验预测和确认了miR-989的靶点。MIR-989只在雌性蚊子卵巢中表达，对血液摄取有反应。注射miR-989 Anagomir会导致卵巢变小，产卵量减少。5-HTR1被证明是miR-989的靶点。MiR-989基因缺失导致5-HTR1表达上调。5-HTR1基因的敲除挽救了miR-989沉默导致的不良产卵。因此，miR-989可能通过靶向5-HTR1在雌性生殖中发挥重要作用。我们发现miR-989以5-HTR1为靶标，参与雌性蚊子生殖的调节。这些发现扩大了我们对与繁殖相关的miRNAs的理解，并促进了新的蚊子控制策略。网上版载有补充材料，可在10.1186/s13071-023-05957-0查阅。
Female mosquitoes need a blood meal after mating for their eggs to develop, and this behavior leads to the spread of pathogens. Therefore, understanding the molecular regulation of reproduction in female mosquitoes is essential to control mosquito vector populations. In this study, we reported that microRNA-989 (miR-989), which targets 5-HTR1 (encoding secreted 5-hydroxytryptamine receptor1), is essential for mosquito reproduction. The spatiotemporal expression profile of miR-989 was detected using quantitative real-time reverse transcription PCR (RT-qPCR). miR-989 antagomirs and antagomir-negative control (NC) were designed and synthesized to knock down the expression of endogenous miR-989 in female mosquitoes. RNA sequencing was used to analyze the ovarian response to miR-989 deletion. The targets of miR-989 were predicted and confirmed using RNAhybrid and dual-luciferase assays. miR-989 is exclusively expressed in female mosquito ovaries and responds to blood feeding. Injection of the miR-989 antagomir resulted in smaller ovaries and reduced egg production. 5-HTR1 was demonstrated as a target of miR-989. The deletion of miR-989 contributed to the upregulation of 5-HTR1 expression. Knockdown of 5-HTR1 rescued the adverse egg production caused by miR-989 silencing. Thus, miR-989 might play an essential role in female reproduction by targeting 5-HTR1. We found that miR-989 targets 5-HTR1 and participates in the regulation of reproduction in female mosquitoes. These findings expand our understanding of reproduction-related miRNAs and promote new control strategies for mosquitoes. The online version contains supplementary material available at 10.1186/s13071-023-05957-0.
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影响因子：
16
作者：
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通讯作者：
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作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1416278111
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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