Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function.
Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function.
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DOI：
10.1093/nar/gkq690
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发表时间：
2010-12
影响因子：
14.9
通讯作者：
Baserga SJ
中科院分区：
文献类型：
--
作者：
Bleichert F;Baserga SJ
In all three kingdoms of life, nucleotides in ribosomal RNA (rRNA) are post-transcriptionally modified. One type of chemical modification is 2′-O-ribose methylation, which is, in eukaryotes and archaea, performed by box C/D small ribonucleoproteins (box C/D sRNPs in archaea) and box C/D small nucleolar ribonucleoproteins (box C/D snoRNPs in eukaryotes), respectively. Recently, the first structure of any catalytically active box C/D s(no)RNP determined by electron microscopy and single particle analysis surprisingly demonstrated that they are dimeric RNPs. Mutational analyses of the Nop5 protein interface suggested that di-sRNP formation is also required for the in vitro catalytic activity. We have now analyzed the functional relevance of the second interface, the sRNA interface, within the box C/D di-sRNP. Mutations in conserved sequence elements of the sRNA, which allow sRNP assembly but which severely interfere with the catalytic activity of box C/D sRNPs, prevent formation of the di-sRNP. In addition, we can observe the dimeric box C/D sRNP architecture with a different box C/D sRNP, suggesting that this architecture is conserved. Together, these results provide further support for the functional relevance of the di-sRNP architecture and also provide a structural explanation for the observed defects in catalysis of 2′-O-ribose methylation.
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DOI：
10.1126/science.1176099
发表时间：
2009-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bleichert F;Gagnon KT;Brown BA 2nd;Maxwell ES;Leschziner AE;Unger VM;Baserga SJ
通讯作者：
Baserga SJ
影响因子：
5.3
作者：
Watkins, NJ;Dickmanns, A;Lührmann, R
通讯作者：
Lührmann, R
影响因子：
64.5
作者：
Watkins, NJ;Ségault, V;Lührmann, R
通讯作者：
Lührmann, R
影响因子：
5.6
作者：
Rashid, R;Aittaleb, M;Li, H
通讯作者：
Li, H
DOI：
10.1038/nsb905
发表时间：
2003-04-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Aittaleb, M;Rashid, R;Li, H
通讯作者：
Li, H