Unraveling the Intertwined Effect of pH on Helicobacter pylori Motility and the Microrheology of the Mucin-Based Medium It Swims in.
Unraveling the Intertwined Effect of pH on Helicobacter pylori Motility and the Microrheology of the Mucin-Based Medium It Swims in.
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DOI：
10.3390/microorganisms11112745
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发表时间：
2023-11-10
期刊：
影响因子：
4.5
通讯作者：
Bansil R
中科院分区：
文献类型：
--
作者：
Su-Arcaro C;Liao W;Bieniek K;Constantino MA;Decker SM;Turner BS;Bansil R
胃病原体幽门螺杆菌必须在粘液层中游过2至7的pH梯度以定殖胃上皮。本课题组先前的研究表明，猪胃粘蛋白（PGM）在pH < 4的酸性条件下形成凝胶，而在pH < 4的酸性条件下形成凝胶。幽门螺杆菌不能在凝胶中游动，尽管它们的鞭毛可以旋转。pH值的改变既影响胃粘蛋白的流变学特性，也影响H. pylori以及它们的抗pH敏感受体。为了阐明酸性pH值对粘蛋白基粘液的粘弹性以及细菌的鞭毛马达和化学受体的这些相互交织的影响，我们比较了H. pylori在PGM中与在布鲁氏菌肉汤（BB 10）中在不同pH值下的比较，用相差显微镜跟踪细菌的运动。结果表明，游泳速度的分布和其他特征的细菌轨迹表现出pH依赖性的差异，在两种介质中。BB 10的游泳速度在pH 4时出现峰值，而PGM在较高的pH 5时出现不太明显的峰值。在所有pH值下，与PGM相比，细菌在BB10中游得更快并且具有更长的净位移。当细菌在pH < 4时被粘在PGM凝胶中时，它们在BB10中在这些酸性pH值下游动，尽管速度降低。降低pH值导致运动细菌的比例降低，速度更快的游泳者对速度和净位移的轨迹分布的贡献减少。在BB10中，身体旋转速率弱地依赖于pH，而在固定在低pH凝胶中的PGM细菌能够机械感测并且旋转得更快。细菌可以以各种方式粘在凝胶中，包括鞭毛缠绕在凝胶的纤维中或细胞体粘在凝胶上。我们的研究结果表明，在BB10中，游泳是最佳的pH值4，反映了pH值传感的反pH战术受体和H+泵的鞭毛电机的影响的综合效应，而随着pH值的降低和凝胶化的PGM粘度的增加低于pH 4导致游泳速度进一步降低，最佳的游泳pH值5和固定化的细菌低于pH 4。
The gastric pathogen, Helicobacter pylori bacteria have to swim across a pH gradient from 2 to 7 in the mucus layer to colonize the gastric epithelium. Previous studies from our group have shown that porcine gastric mucin (PGM) gels at an acidic pH < 4, and H. pylori bacteria are unable to swim in the gel, although their flagella rotate. Changing pH impacts both the rheological properties of gastric mucin and also influences the proton (H+)-pumped flagellar motors of H. pylori as well as their anti-pH sensing receptors. To unravel these intertwined effects of acidic pH on both the viscoelastic properties of the mucin-based mucus as well as the flagellar motors and chemo-receptors of the bacterium, we compared the motility of H. pylori in PGM with that in Brucella broth (BB10) at different pH values using phase contrast microscopy to track the motion of the bacteria. The results show that the distribution of swimming speeds and other characteristics of the bacteria trajectories exhibit pH-dependent differences in both media. The swimming speed exhibits a peak at pH 4 in BB10, and a less pronounced peak at a higher pH of 5 in PGM. At all pH values, the bacteria swam faster and had a longer net displacement in BB10 compared to PGM. While the bacteria were stuck in PGM gels at pH < 4, they swam at these acidic pH values in BB10, although with reduced speed. Decreasing pH leads to a decreased fraction of motile bacteria, with a decreased contribution of the faster swimmers to the distributions of speeds and net displacement of trajectories. The body rotation rate is weakly dependent on pH in BB10, whereas in PGM bacteria that are immobilized in the low pH gel are capable of mechano-sensing and rotate faster. Bacteria can be stuck in the gel in various ways, including the flagella getting entangled in the fibers of the gel or the cell body being stuck to the gel. Our results show that in BB10, swimming is optimized at pH4, reflecting the combined effects of pH sensing by anti-pH tactic receptors and impact on H+ pumping of flagellar motors, while the increase in viscosity of PGM with decreasing pH and gelation below pH 4 lead to further reduction in swimming speed, with optimal swimming at pH 5 and immobilization of bacteria below pH 4.
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影响因子：
13.6
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