Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo.
Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo.
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体内精子发生过程中自噬体膜生物发生的亚细胞证据
DOI：
10.3389/fphys.2016.00470
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发表时间：
2016
影响因子：
4
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Huang Y;Yang P;Liu T;Chen H;Chu X;Ahmad N;Zhang Q;Li Q;Hu L;Liu Y;Chen Q
尽管自噬体的形成引起了大量的关注，但自噬体膜的起源和来源仍未得到解决。本研究采用透射电镜（TEM）、免疫印迹（Western blots）和免疫组化（immunohistochemistry）技术对中华鳖精子发生过程中自噬体膜生物发生的体内亚细胞证据进行了研究。睾丸表达LC3-II蛋白，该蛋白位于不同分化阶段的精细胞内，表明细胞自噬活跃。透射电镜显示，精子内发育了大量自噬体。许多内质网（ER）转移到一个特殊的“菊花中心”（CFC），在这个中心形成并延伸了几层双层隔离膜（IM）。伸长的胞浆总是吞没一些细胞质和各种结构。狭窄的小管连接多个ER和CFC的末端。致密核精子细胞的CFC比颗粒状精子细胞更发达。IM也可以从单个ER转换而来。有时，从跨高尔基网络扩展的IM包裹了不同的结构。精子发生时，精子的质膜内陷形成囊泡，囊泡分布在CFC周围的各种核内体中。所有这些细胞证据表明，在体内，IM主要是在精子发生过程中由ER在分化的精子中产生的CFC发展起来的。来自高尔基复合体、质膜和核内体的囊泡也可能是自噬体膜的来源。
Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. TEM showed that numerous autophagosomes were developed inside spermatids. Many endoplasmic reticulum (ER) were transferred into a special “Chrysanthemum flower center” (CFC) in which several double-layer isolation membranes (IM) were formed and extended. The elongated IM always engulfed some cytoplasm and various structures. Narrow tubules connected the ends of multiple ER and the CFC. The CFC was more developed in spermatids with compact nuclei than in spermatids with granular nuclei. An IM could also be transformed from a single ER. Sometimes an IM extended from a trans-Golgi network and wrapped different structures. The plasma membrane of the spermatid invaginated to form vesicles that were distributed among various endosomes around the CFC during spermiogenesis. All this cellular evidence suggests that, in vivo, IM was developed mainly by CFC produced from ER within differentiating spermatids during spermiogenesis. Vesicles from Golgi complexes, plasma membranes and endosomes might also be the sources of the autophagosome membrane.
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影响因子：
64.8
作者：
Diao, Jiajie;Liu, Rong;Rong, Yueguang;Zhao, Minglei;Zhang, Jing;Lai, Ying;Zhou, Qiangjun;Wilz, Livia M.;Li, Jianxu;Vivona, Sandro;Pfuetzner, Richard A.;Brunger, Axel T.;Zhong, Qing
通讯作者：
Zhong, Qing
DOI：
10.1083/jcb.152.4.657
发表时间：
2001-02-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mizushima N;Yamamoto A;Hatano M;Kobayashi Y;Kabeya Y;Suzuki K;Tokuhisa T;Ohsumi Y;Yoshimori T
通讯作者：
Yoshimori T
影响因子：
21.3
作者：
Hayashi-Nishino, Mitsuko;Fujita, Naonobu;Yamamoto, Akitsugu
通讯作者：
Yamamoto, Akitsugu
影响因子：
7.5
作者：
Kraft, Claudine;Martens, Sascha
通讯作者：
Martens, Sascha
影响因子：
3.3
作者：
Fujita, Naonobu;Hayashi-Nishino, Mitsuko;Yoshimori, Tamotsu
通讯作者：
Yoshimori, Tamotsu