Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion.
Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion.
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DOI：
10.1038/s41467-023-36762-z
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发表时间：
2023-03-02
影响因子：
16.6
通讯作者：
Orth, Kim
中科院分区：
文献类型：
--
作者：
Kaval, Karan Gautam;Chimalapati, Suneeta;Siegel, Sara D.;Garcia, Nalleli;Jaishankar, Jananee;Dalia, Ankur B.;Orth, Kim
有人提出，细菌膜蛋白可以通过一种称为转座的过程合成并插入到膜上，该过程涉及编码基因的膜结合，随后是偶联转录、翻译和膜插入。在这里，我们提供了支持病原体副溶血性弧菌利用插入组装其III型分泌系统(T3SS2)，向宿主细胞注入毒力因子的证据。我们提出了一个两步插入过程，其中膜结合的共组分受体(VtrA/VtrC)首先被胆汁酸激活，导致其靶基因vtrB的膜结合和表达，该基因位于T3SS2致病岛上。T3SS2的跨膜转录激活因子VtrB诱导T3SS2结构成分及其效应物的定位表达和膜组装。我们推测，所提出的插入过程可能被其他肠道细菌用来响应细胞外信号而有效地组装膜结合的分子复合体。有人提出，细菌的膜蛋白可以通过膜相关基因的‘插入’，即同时转录、翻译和膜插入来产生。在这里，Kaval等人。提供证据支持副溶血性弧菌利用插入作用组装用于向宿主细胞注入毒力因子的跨膜复合体(III型分泌系统)。
It has been proposed that bacterial membrane proteins may be synthesized and inserted into the membrane by a process known as transertion, which involves membrane association of their encoding genes, followed by coupled transcription, translation and membrane insertion. Here, we provide evidence supporting that the pathogen Vibrio parahaemolyticus uses transertion to assemble its type III secretion system (T3SS2), to inject virulence factors into host cells. We propose a two-step transertion process where the membrane-bound co-component receptor (VtrA/VtrC) is first activated by bile acids, leading to membrane association and expression of its target gene, vtrB, located in the T3SS2 pathogenicity island. VtrB, the transmembrane transcriptional activator of T3SS2, then induces the localized expression and membrane assembly of the T3SS2 structural components and its effectors. We hypothesize that the proposed transertion process may be used by other enteric bacteria for efficient assembly of membrane-bound molecular complexes in response to extracellular signals. It has been proposed that bacterial membrane proteins may be produced via ‘transertion’, or concurrent transcription, translation and membrane insertion from membrane-associated genes. Here, Kaval et al. provide evidence supporting that Vibrio parahaemolyticus uses transertion to assemble a transmembrane complex (type III secretion system) used to inject virulence factors into host cells.
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影响因子：
3.9
作者：
Norris, V;Alexandre, S;Wiggins, P
通讯作者：
Wiggins, P
影响因子：
3.7
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影响因子：
5.6
作者：
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通讯作者：
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16
作者：
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通讯作者：
Amster-Choder, Orna
影响因子：
3.2
作者：
MILLER, VL;DIRITA, VJ;MEKALANOS, JJ
通讯作者：
MEKALANOS, JJ