MALDI/post ionization-ion mobility mass spectrometry of noncovalent complexes of dopamine receptors' epitopes.
MALDI/post ionization-ion mobility mass spectrometry of noncovalent complexes of dopamine receptors' epitopes.
复制标题
多巴胺受体表位的非共价复合物的MALDI/电离后离子迁移率质谱。
DOI：
10.1021/pr301004w
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发表时间：
2013-04-05
影响因子：
4.4
通讯作者：
Schultz JA
中科院分区：
文献类型：
--
作者：
Woods AS;Jackson SN;Lewis EK;Egan T;Muller L;Tabet JC;Schultz JA
参与受体异聚体形成的蛋白质结构域是无序的，并且富含形成非共价复合物（NCX）所必需的氨基酸。我们目前的质谱NCX数据从蛋白质和蛋白质受体的表位通过结合离子迁移率（IM）和MALDI。我们的主要焦点是NCX参与多巴胺D2（D2 R）和腺苷A2 A受体（A2 AR）以及D2 R和烟碱（NR）受体亚基的表位之间的异聚体形成。的IM数据产量的单电荷的NCX的气相构象上的信息，观察到直接从MALDI或作为共解吸的中性随后后电离的时间延迟的准分子激光脉冲直接到一部分的中性羽由MALDI解吸激光器创建。基质/表位干燥液滴表面的成像质谱显示，发现酸性和碱性表位及其NCX在小至25×50微米的区域内空间排列。质子化和阳离子化NCX和表位的相对丰度的细微差异在液滴的富钠外边界附近的空间区域中测量。
Protein domains involved in receptor heteromer formation are disordered and rich in the amino acids necessary for the formation of noncovalent complexes (NCX). We present mass spectral NCX data from proteins and protein receptors' epitopes obtained by combining ion mobility (IM) and MALDI. Our main focus are NCX involved in heteromer formation occurring between epitopes of the Dopamine D2 (D2R) and Adenosine A2A receptors (A2AR) as well as D2R and a nicotinic (NR) receptors' subunit. The IM data yield information on the gas phase conformation of the singly charged NCX which are observed either directly from MALDI or as co-desorbed neutrals which are subsequently post-ionized by a time-delayed excimer laser pulse directed onto a portion of the neutral plume created by the MALDI desorption laser. Imaging mass spectrometry of the matrix/epitope dried droplet surface shows that the acidic and basic epitopes and their NCX are found to be spatially collocated within regions as small as 25×50 microns. Subtle differences in the relative abundance of protonated and cationized NCX and epitopes are measured in spatial regions near the sodium rich outer border of the droplet.
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影响因子：
2.8
作者：
Francesco AL;Kjell F;Amina W;Susanna G;Diego G
通讯作者：
Diego G
DOI：
10.1038/npp.2008.144
发表时间：
2009-03
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
Azdad K;Gall D;Woods AS;Ledent C;Ferré S;Schiffmann SN
通讯作者：
Schiffmann SN
DOI：
10.1016/j.jasms.2008.06.023
发表时间：
2008-10
影响因子：
3.2
作者：
Jackson SN;Moyer SC;Woods AS
通讯作者：
Woods AS
影响因子：
4.4
作者：
Jackson, SN;Wang, HYJ;Woods, AS
通讯作者：
Woods, AS
DOI：
10.1016/j.jasms.2007.04.015
发表时间：
2007-08-01
影响因子：
3.2
作者：
Thompson, Matthew S.;Cui, Weidong;Reilly, James P.
通讯作者：
Reilly, James P.