Identification and Functional Characterization of a Microtubule-Associated Protein, GhCLASP2, From Upland Cotton (Gossypium hirsutum L.)
Identification and Functional Characterization of a Microtubule-Associated Protein, GhCLASP2, From Upland Cotton (Gossypium hirsutum L.)
复制标题
来自陆地棉 (Gossypium hirsutum L.) 的微管相关蛋白 GhCLASP2 的鉴定和功能表征
DOI：
10.3389/fpls.2018.00882
复制
发表时间：
2018-06
影响因子：
5.6
通讯作者：
Sun Jie
中科院分区：
文献类型：
--
作者：
Zhu Shou-Hong;Xue Fei;Li Yan-Jun;Liu Feng;Zhang Xin-Yu;Zhao Lan-Jie;Sun Yu-Qiang;Zhu Qian-Hao;Sun Jie
细胞质连接相关蛋白（CLASPs）是微管相关蛋白（MAPs），参与微管（MTs）动力学的调控，在植物生长和发育中起重要作用。在这项研究中，我们鉴定了棉花的CLASP基因
Cytoplasmic linker-associated proteins (CLASPs) are microtubule-associated proteins (MAPs) involved in regulation of dynamics of microtubules (MTs) that play an important role in plant growth and development. In this study, we identified cotton CLASP genes and investigated the function of GhCLASP2. GhCLASP2 was mainly expressed in stem and developing fibers, especially in fibers of the secondary cell wall deposition stage. Ectopic expression of GhCLASP2 in Arabidopsis increased the branching number of leaf trichomes and rescued the defective phenotypes of clasp-1. In cotton, overexpression of GhCLASP2 increased fiber strength, probably related to enhanced expression levels of tubulin, cellulose synthase, and expansin genes. Suppression of GhCLASP2 caused shorter internodes and semi-dwarfism, abnormal flower stigma, aborted anthers without pollen grains, and sterility. These changed phenotypes were similar to those observed in the Arabidopsis clasp-1 mutant. GhCLASP2 was co-localized with MTs according to transient experiment. These results suggest that GhCLASP2 functions similarly as AtCLASP, acting as a MAP and controlling cotton growth and development by regulating MTs.
登录
查看更多内容
DOI：
10.1083/jcb.200912134
发表时间：
2010-05-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Stramer B;Moreira S;Millard T;Evans I;Huang CY;Sabet O;Milner M;Dunn G;Martin P;Wood W
通讯作者：
Wood W
RAC1和GSK3BETA在迁移上皮细胞中对微管对微管的扣发的空间调节。
影响因子：
7.8
作者：
Wittmann, Torsten;Waterman-Storer, Clare M
通讯作者：
Waterman-Storer, Clare M
DOI：
10.1083/jcb.200405094
发表时间：
2005-01-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mimori-Kiyosue Y;Grigoriev I;Lansbergen G;Sasaki H;Matsui C;Severin F;Galjart N;Grosveld F;Vorobjev I;Tsukita S;Akhmanova A
通讯作者：
Akhmanova A
影响因子：
5.6
作者：
Brumbarova T;Ivanov R
通讯作者：
Ivanov R
影响因子：
11.6
作者：
Dixit, R;Cyr, R
通讯作者：
Cyr, R