From molecules to myofibers: multiscale imaging of the myocardium.
From molecules to myofibers: multiscale imaging of the myocardium.
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从分子到肌纤维：心肌的多尺度成像。
DOI：
10.1007/s12265-011-9284-0
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发表时间：
2011-08
影响因子：
3.4
通讯作者：
Sosnovik, David E.
中科院分区：
文献类型：
--
作者：
Goergen, Craig J.;Sosnovik, David E.
心脏的病理学可以在几个尺度上进行检查，从分子到宏观。传统上，基于荧光的技术如流式细胞术已被用于在分子、细胞和微观水平上研究心肌。然而，磁共振成像（MRI）的最新进展使得在体内无创地对心肌中的某些细胞和分子事件进行成像成为可能。此外，弥散MRI已被用于成像心肌纤维结构和微结构在完整的心脏。特别是弥散磁共振纤维束成像，为健康和疾病的心肌显微结构提供了新的见解。最近的发展也取得了荧光成像，使其有可能成像的小动物的心脏中的荧光探针在体内非侵入性。此外，已经开发了用于对小鼠心脏进行体内荧光断层扫描的技术。MRI和荧光成像的这些进展使得心肌中的事件可以在几个尺度上成像，将分子变化与微观结构的改变以及微观结构变化与总体功能联系起来。从而获得心肌病理生理学的完整和综合的图像。这种多尺度方法不仅在临床前研究中，而且最终在临床竞技场中具有重要价值。
Pathology in the heart can be examined at several scales, ranging from the molecular to the macroscopic. Traditionally, fluorescence-based techniques such as flow cytometry have been used to study the myocardium at the molecular, cellular, and microscopic levels. Recent advances in magnetic resonance imaging (MRI), however, have made it possible to image certain cellular and molecular events in the myocardium noninvasively in vivo. In addition, diffusion MRI has been used to image myocardial fiber architecture and microstructure in the intact heart. Diffusion MRI tractography, in particular, is providing novel insights into myocardial microsctructure in both health and disease. Recent developments have also been made in fluorescence imaging, making it possible to image fluorescent probes in the heart of small animals non-invasively in vivo. Moreover, techniques have been developed to perform in vivo fluorescence tomography of the mouse heart. These advances in MRI and fluorescence imaging allow events in the myocardium to be imaged at several scales linking molecular changes to alterations in microstructure and microstructural changes to gross function. A complete and integrated picture of pathophysiology in the myocardium is thus obtained. This multiscale approach has the potential to be of significant value not only in preclinical research but, ultimately, in the clinical arena as well.
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