Polycomb-group recruitment to a Drosophila target gene is the default state that is inhibited by a transcriptional activator.
Polycomb-group recruitment to a Drosophila target gene is the default state that is inhibited by a transcriptional activator.
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DOI：
10.1126/sciadv.abg1556
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发表时间：
2021-07
期刊：
影响因子：
13.6
通讯作者：
Jones RS
中科院分区：
文献类型：
--
作者：
Ghotbi E;Ye P;Ervin T;Kum A;Benes J;Jones RS
多梳蛋白在默认情况下被招募到目标基因上，不需要转录因子的帮助。聚梳组蛋白(Polycomb-group，PcG)是一种表观遗传调节因子，在转录因子对靶基因进行转录抑制后，维持其转录抑制。PCG靶基因在一些细胞中被抑制，但在另一些细胞中却是活跃的。因此，必须存在一种机制，使PcG蛋白区分抑制状态和活动状态，并仅在被抑制的靶基因上组装抑制染色质环境。在这里，我们提出的实验证据表明，果蝇PcG靶基因GIGN(GT)的抑制状态并不是通过抑制物的存在来确定的。相反，在GT从头开始建立PcG介导的沉默是默认状态，这是由于存在激活剂或辅助激活剂而阻止的，这可能会抑制多聚梳抑制复合体2(PRC2)的催化活性。
Polycomb-group proteins are recruited to a target gene by default and do not require assistance from transcription factors. Polycomb-group (PcG) proteins are epigenetic regulators that maintain the transcriptional repression of target genes following their initial repression by transcription factors. PcG target genes are repressed in some cells, but active in others. Therefore, a mechanism must exist by which PcG proteins distinguish between the repressed and active states and only assemble repressive chromatin environments at target genes that are repressed. Here, we present experimental evidence that the repressed state of a Drosophila PcG target gene, giant (gt), is not identified by the presence of a repressor. Rather, de novo establishment of PcG-mediated silencing at gt is the default state that is prevented by the presence of an activator or coactivator, which may inhibit the catalytic activity of Polycomb-repressive complex 2 (PRC2).
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影响因子：
14.9
作者：
Contrino S;Smith RN;Butano D;Carr A;Hu F;Lyne R;Rutherford K;Kalderimis A;Sullivan J;Carbon S;Kephart ET;Lloyd P;Stinson EO;Washington NL;Perry MD;Ruzanov P;Zha Z;Lewis SE;Stein LD;Micklem G
通讯作者：
Micklem G
富含GC的序列元件在哺乳动物ES细胞中募集PRC2。
影响因子：
4.5
作者：
Mendenhall EM;Koche RP;Truong T;Zhou VW;Issac B;Chi AS;Ku M;Bernstein BE
通讯作者：
Bernstein BE
影响因子：
16
作者：
Kanhere A;Viiri K;Araújo CC;Rasaiyaah J;Bouwman RD;Whyte WA;Pereira CF;Brookes E;Walker K;Bell GW;Pombo A;Fisher AG;Young RA;Jenner RG
通讯作者：
Jenner RG
DOI：
10.1016/j.cub.2009.02.055
发表时间：
2009-04-28
期刊：
Current biology : CB
影响因子：
--
作者：
Chopra VS;Hong JW;Levine M
通讯作者：
Levine M
影响因子：
2.4
作者：
Kosman, D;Small, S;Reinitz, J
通讯作者：
Reinitz, J