Engineering aligned human cardiac muscle using developmentally inspired fibronectin micropatterns.
Engineering aligned human cardiac muscle using developmentally inspired fibronectin micropatterns.
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DOI：
10.1038/s41598-021-87550-y
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发表时间：
2021-06-01
影响因子：
4.6
通讯作者：
Feinberg AW
中科院分区：
文献类型：
--
作者：
Batalov I;Jallerat Q;Kim S;Bliley J;Feinberg AW
心脏二维组织是利用基于胚胎心脏富含纤连蛋白的细胞外基质（ECM）的仿生微图案进行工程设计的。这种受发育启发的体外方法的目标是识别早期四腔脊椎动物心脏微环境中驱动心肌细胞组织和排列的细胞-细胞和细胞-ECM 相互作用。为了测试这一点，创建了基于胚胎鸡心肌纤连蛋白共聚焦成像的仿生微图案，并与用 2 或 20 µm 宽纤连蛋白线设计的对照微图案进行比较。结果表明，胚胎鸡心肌细胞在仿生微图案上具有独特的密度依赖性排列，该排列部分由 N-钙粘蛋白介导，这表明细胞-细胞和细胞-ECM 相互作用在排列心肌的形成中发挥着重要作用。人诱导多能干细胞衍生的心肌细胞在仿生微图案上也表现出密度依赖性排列，但总体组织较差。有趣的是，添加成人心脏成纤维细胞和用 T3 激素调理均显示可以增加人类心肌细胞的排列。总而言之，这些结果表明，心肌细胞成熟状态、心肌细胞-心肌细胞和心肌细胞-成纤维细胞相互作用以及心肌细胞-ECM相互作用都可以在体外工程各向异性心脏组织时发挥作用，并提供有关这些因素如何影响体内心脏发生的见解。
Cardiac two-dimensional tissues were engineered using biomimetic micropatterns based on the fibronectin-rich extracellular matrix (ECM) of the embryonic heart. The goal of this developmentally-inspired, in vitro approach was to identify cell–cell and cell-ECM interactions in the microenvironment of the early 4-chambered vertebrate heart that drive cardiomyocyte organization and alignment. To test this, biomimetic micropatterns based on confocal imaging of fibronectin in embryonic chick myocardium were created and compared to control micropatterns designed with 2 or 20 µm wide fibronectin lines. Results show that embryonic chick cardiomyocytes have a unique density-dependent alignment on the biomimetic micropattern that is mediated in part by N-cadherin, suggesting that both cell–cell and cell-ECM interactions play an important role in the formation of aligned myocardium. Human induced pluripotent stem cell-derived cardiomyocytes also showed density-dependent alignment on the biomimetic micropattern but were overall less well organized. Interestingly, the addition of human adult cardiac fibroblasts and conditioning with T3 hormone were both shown to increase human cardiomyocyte alignment. In total, these results show that cardiomyocyte maturation state, cardiomyocyte-cardiomyocyte and cardiomyocyte-fibroblast interactions, and cardiomyocyte-ECM interactions can all play a role when engineering anisotropic cardiac tissues in vitro and provides insight as to how these factors may influence cardiogenesis in vivo.
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DOI：
10.1126/science.1177350
发表时间：
2009-10-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Domian IJ;Chiravuri M;van der Meer P;Feinberg AW;Shi X;Shao Y;Wu SM;Parker KK;Chien KR
通讯作者：
Chien KR
影响因子：
9.8
作者：
Astrof S;Hynes RO
通讯作者：
Hynes RO
DOI：
10.1016/j.bbamcr.2015.11.005
发表时间：
2016-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Bedada FB;Wheelwright M;Metzger JM
通讯作者：
Metzger JM
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
48
作者：
Burridge, Paul W.;Matsa, Elena;Shukla, Praveen;Lin, Ziliang C.;Churko, Jared M.;Ebert, Antje D.;Lan, Feng;Diecke, Sebastian;Huber, Bruno;Mordwinkin, Nicholas M.;Plews, Jordan R.;Abilez, Oscar J.;Cui, Bianxiao;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.