The effect of opioids and their antagonists on the nocifensive response of Caenorhabditis elegans to noxious thermal stimuli.
The effect of opioids and their antagonists on the nocifensive response of Caenorhabditis elegans to noxious thermal stimuli.
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DOI：
10.1007/s10158-010-0099-5
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发表时间：
2009-12
影响因子：
--
通讯作者：
Sood, R.
中科院分区：
文献类型：
--
作者：
Nieto-Fernandez, F.;Andrieux, S.;Idrees, S.;Bagnall, C.;Pryor, S. C.;Sood, R.
阿片类药物调节脊椎动物的伤害感受。这一点在一些无脊椎动物模型中也得到了证实。在此，阿片类吗啡和阿片类神经肽内吗啡肽1和2对秀丽隐杆线虫的热回避（Tav）行为的影响进行了探讨。野生型C.使用M9缓冲液从NGM板收集线虫N2，并暴露于浓度在10−8和10−4 M（2.5 pmol/mg至25 nmol/mg）之间的吗啡和内吗啡1和2 30分钟，并测试Tav。阿片受体拮抗剂纳洛酮和CTOP与药物组合进行了测试。47%的吗啡暴露蠕虫表现出I类反应，而对照组为76%（P < 0.001）。内吗啡肽1和2也导致I类反应的统计学显著降低，分别为36%和39%。这些作用被纳洛酮和CTOP逆转。C. elegans是由阿片类调节的。
Opiates modulate nociception in vertebrates. This has also been demonstrated in a number of invertebrate models. Herein, the effect of the opiate morphine and opioid neuropeptides Endomorphin 1 and 2 on the thermal avoidance (Tav) behavior of Caenorhabditis elegans is explored. Adult wild-type C. elegans N2 were collected from NGM plates using M9 buffer and exposed to morphine and endomorphine 1 and 2 in concentrations between 10−8 and 10−4 M (2.5 pmol/mg to 25 nmol/mg) for 30 min and tested for Tav. The opioid receptor antagonists Naloxone and CTOP were tested in combination with the drugs. Forty-seven percentage of the morphine exposed worms exhibited a class I response versus 76% of the control group (P < 0.001). Endomorphin 1 and 2 also caused a statistically significant reduction in class I responses, 36 and 39%, respectively. These effects were reversed with Naloxone and CTOP. Thermonocifensive behavior in C. elegans is modulated by opioids.
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影响因子：
4.8
作者：
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