Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation.
Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation.
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抑制 nbe-miR166h-p5 可减轻本塞姆氏烟草上马铃薯 X 病毒的叶片黄化症状，并减少病毒积累。
DOI：
10.1111/mpp.12717
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发表时间：
2018-11
影响因子：
4.9
通讯作者：
Yan F
中科院分区：
文献类型：
--
作者：
Wang S;Cui W;Wu X;Yuan Q;Zhao J;Zheng H;Lu Y;Peng J;Lin L;Chen J;Yan F
MicroRNAs （miRNAs）在植物发育中起着至关重要的作用。越来越多的证据表明，受病毒感染的植物中mirna表达的改变有助于病毒症状的发展。本研究利用Illumina Solexa测序技术分析了本菌烟草（Nicotiana benthamiana）对马铃薯病毒X （PVX）的mirna表达变化。在21个受到显著影响的mirna中，nbe‐miR166h‐p5与病毒症状密切相关。利用基于烟草摇铃病毒的miRNA抑制（VbMS）系统，我们发现nbe‐miR166h‐p5的抑制使植物叶片变成深绿色，叶绿素含量增加。当PVX接种于nbe‐miR166h‐p5‐抑制的植株上时，PVX的叶片黄化症状明显减弱，叶绿素含量下降较少，PVX的积累减少。nbe‐miR166h‐p5在芜菁花叶病毒（TuMV）侵染植物中也被上调表达，其抑制作用减弱了TuMV的叶片泛黄症状，减少了病毒的积累。我们确定了nbe‐miR166h‐p5的三个潜在靶点。结果表明，nbe‐miR166h‐p5与PVX和TuMV的症状相关，为miRNA与病毒感染之间的关系提供了有用的信息。
MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus‐infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illumina Solexa sequencing. One of the 21 miRNAs significantly affected, nbe‐miR166h‐p5, was closely associated with viral symptoms. Using the Tobacco rattle virus‐based miRNA suppression (VbMS) system, we found that the suppression of nbe‐miR166h‐p5 in plants caused leaves to turn dark green with increased chlorophyll. When PVX was inoculated on nbe‐miR166h‐p5‐suppressed plants, the leaf yellowing symptom of PVX was largely attenuated with less reduction in chlorophyll content, and the accumulation of PVX was decreased. nbe‐miR166h‐p5 was also up‐regulated in plants infected by Turnip mosaic virus (TuMV), and its suppression attenuated the leaf yellowing symptom of TuMV and decreased viral accumulation. Three potential targets of nbe‐miR166h‐p5 were identified. The results indicate the association of nbe‐miR166h‐p5 with symptoms of PVX and also with those of TuMV, providing useful information on the relationship between miRNA and viral infection.
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影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
4.8
作者：
Amin I;Patil BL;Briddon RW;Mansoor S;Fauquet CM
通讯作者：
Fauquet CM
影响因子：
10.2
作者：
Katiyar-Agarwal S;Jin H
通讯作者：
Jin H
DOI：
10.1073/pnas.0705114104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Bazzini, A. A.;Hopp, H. E.;Asurmendi, S.
通讯作者：
Asurmendi, S.
影响因子：
11.6
作者：
Guo, HS;Xie, Q;Chua, NH
通讯作者：
Chua, NH