Ratiometric coumarin-neutral red (CONER) nanoprobe for detection of hydroxyl radicals.
Ratiometric coumarin-neutral red (CONER) nanoprobe for detection of hydroxyl radicals.
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DOI：
10.1021/ac102874x
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发表时间：
2011-04-01
影响因子：
7.4
通讯作者：
Warner, Isiah M.
中科院分区：
文献类型：
--
作者：
Ganea, Gabriela M.;Kolic, Paulina E.;El-Zahab, Bilal;Warner, Isiah M.
活性氧的过量产生会导致细胞功能的改变，从而导致许多疾病，包括心血管、神经退行性疾病、癌症和衰老。羟基自由基是一种短命自由基，由于其对生物分子的高反应性而被认为非常具有攻击性。在本研究中，开发了一种香豆素-中性红（CONER）纳米探针，用于基于7-羟基香豆素3-羧酸和中性红染料之间的比率荧光信号检测羟基自由基。使用香豆素 3-羧酸共轭聚（N-十一碳烯基-Nε-赖氨酸钠）（C3C-聚-Nε-SUK）作为对羟基自由基具有反应性的部分，制备了含有封装中性红的生物相容性聚丙交酯-共-乙交酯（PLGA）纳米颗粒。 CONER 纳米探针的响应取决于各种参数，例如反应时间和纳米颗粒浓度。与其他活性氧（包括 O2•−、H2O2、1O2 和 OCl−1）相比，该探针对羟基自由基具有选择性。此外，使用 CONER 纳米探针在体外使用暴露于氧化应激的活乳腺癌细胞检测羟基自由基。结果表明，这种纳米探针代表了一种有前途的检测生物系统中羟基自由基的方法。
Excessive production of reactive oxygen species can lead to alteration of cellular functions responsible for many diseases including cardiovascular, neurodegenerative, cancer, and aging. Hydroxyl radical is a short-lived radical which is considered very aggressive due to its high reactivity towards biological molecules. In this study, a COumarin-NEutral Red (CONER) nanoprobe was developed for the detection of hydroxyl radical based on the ratiometric fluorescence signal between 7-hydroxy coumarin 3-carboxylic acid and neutral red dyes. Biocompatible poly-lactide-co-glycolide (PLGA) nanoparticles containing encapsulated neutral red were produced using a coumarin 3-carboxylic acid conjugated poly(sodium N-undecylenyl-Nε-lysinate) (C3C-poly-Nε-SUK) as moiety reactive to hydroxyl radicals. The response of the CONER nanoprobe was dependent on various parameters such as reaction time and nanoparticle concentration. The probe was selective for hydroxyl radicals as compared with other reactive oxygen species including O2•−, H2O2, 1O2, and OCl−1. Furthermore, the CONER nanoprobe was used to detect hydroxyl radicals in vitro using viable breast cancer cells exposed to oxidative stress. The results suggest that this nanoprobe represents a promising approach for the detection of hydroxyl radicals in biological systems.
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影响因子：
2.9
作者：
Newton, GL;Milligan, JR
通讯作者：
Milligan, JR
影响因子：
3.5
作者：
Ganea, Gabriela M.;Fakayode, Sayo O.;Warner, Isiah M.
通讯作者：
Warner, Isiah M.
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Ganea, Gabriela M.;Sabilov, Cristina M.;Warner, Isiah M.
通讯作者：
Warner, Isiah M.
影响因子：
1.6
作者：
Soh, Nobuaki;Makihara, Koji;Imato, Toshihiko
通讯作者：
Imato, Toshihiko