Diagnosis of canine coronavirus infection using nested-PCR.
Diagnosis of canine coronavirus infection using nested-PCR.
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DOI：
10.1016/s0166-0934(99)00134-2
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发表时间：
2000-01
影响因子：
3.1
通讯作者：
Buonavoglia C
中科院分区：
文献类型：
--
作者：
Pratelli A;Buonavoglia D;Martella V;Tempesta M;Lavazza A;Buonavoglia C
本文报道了用聚合酶链反应（PCR）和巢式聚合酶链反应（n-PCR）诊断犬冠状病毒（CCV）感染的结果，并与电镜（EM）和A-72细胞分离病毒等诊断技术进行了比较。对71个患有肠炎的幼仔粪便样本进行了研究。在71份样本中，14份样本PCR检测结果为阳性，而30份样本n-PCR检测结果为阳性。45份样品中仅4份经EM检查检出CCV，30份样品中3份经病毒分离检出N-PCR阳性。
The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported. The study was carried out on 71 faecal samples of pups with enteritis. Of 71 samples examined 14 were positive in PCR, whereas 30 samples resulted positive in the n-PCR assay. CCV was detected by EM examination in only four out of 45 samples, and by virus isolation in three out of 30 samples n-PCR positive.
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影响因子：
2.4
作者：
Tennant BJ;Gaskell RM;Kelly DF;Carter SD;Gaskell CJ
通讯作者：
Gaskell CJ
影响因子：
5.4
作者：
Herrewegh, AAPM;Smeenk, I;de Groot, RJ
通讯作者：
de Groot, RJ
影响因子：
3.1
作者：
Pratelli A;Tempesta M;Greco G;Martella V;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
1.1
作者：
HYATT, AD
通讯作者：
HYATT, AD