Canine coronavirus infection in the dog following oronasal inoculation.
Canine coronavirus infection in the dog following oronasal inoculation.
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DOI：
10.1016/0034-5288(91)90023-h
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发表时间：
1991-07
影响因子：
2.4
通讯作者：
Gaskell CJ
中科院分区：
文献类型：
--
作者：
Tennant BJ;Gaskell RM;Kelly DF;Carter SD;Gaskell CJ
对 10 周龄幼犬经口鼻接种后 14 天的犬冠状病毒 (CCV) 感染发病机制进行了研究。接种后 3 至 11 天出现轻度腹泻，大约与粪便病毒排出同时发生。病毒最初在第 3 天从扁桃体中分离出来，然后在接种后 14 天从小肠和大肠组织中分离出来。还从肝脏和肺中分离出病毒。在任何组织中均未观察到组织学变化，但使用过氧化物酶抗过氧化物酶染色技术检测到了 CCV 抗原，主要在覆盖肠道相关淋巴组织的上皮中检测到。第10天首次检测到病毒中和抗体。接种后3天血浆中首次检测到特异性抗CCV IgM，第4至7天检测到IgG。十二指肠分泌物中检测到少量抗CCV IgG、IgM和IgA，但胆汁中未检测到。
The pathogenesis of canine coronavirus (CCV) infection in 10-week-old puppies was studied up to 14 days after oronasal inoculation. Mild diarrhoea was seen from three to 11 days after inoculation, approximately coincident with faecal virus shedding. Virus was initially isolated from the tonsils on day 3, and then from both small and large intestinal tissues up to 14 days after inoculation. Virus was also isolated from liver and lung. Histological changes were not seen in any tissues, but CCV antigen was detected, using a peroxidase antiperoxidase staining technique, mainly in epithelium overlying gut-associated lymphoid tissue. Virus neutralising antibody was first detected on day 10. Specific anti-CCV IgM was first detected in plasma three days after inoculation and IgG on days 4 to 7. Small amounts of anti-CCV IgG, IgM and IgA were detected in duodenal secretion, but none in bile.
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影响因子：
3.8
作者：
JARRETT, O;LAIRD, HM;HAY, D
通讯作者：
HAY, D
影响因子：
2.4
作者：
GASKELL, CJ;GASKELL, RM;WOOLDRIDGE, MJA
通讯作者：
WOOLDRIDGE, MJA
影响因子：
3.1
作者：
BOHL, EH;SAIF, LJ
通讯作者：
SAIF, LJ
影响因子：
2.2
作者：
MACARTNEY, L;ALMASHAT, RR;MCCANDLISH, IAP
通讯作者：
MCCANDLISH, IAP
影响因子：
2.2
作者：
NEWTON, CM;NEWELL, DG;BASKERVILLE, M
通讯作者：
BASKERVILLE, M