Partial proteomic analysis of brown widow spider (Latrodectus geometricus) venom to determine the biological activities.
Partial proteomic analysis of brown widow spider (Latrodectus geometricus) venom to determine the biological activities.
复制标题
DOI：
10.1016/j.toxcx.2020.100062
复制
发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Daduang S
中科院分区：
文献类型：
--
作者：
Khamtorn P;Rungsa P;Jangpromma N;Klaynongsruang S;Daduang J;Tessiri T;Daduang S
蜘蛛用毒液来防御和捕捉猎物。这些毒液含有生物活性化合物的混合物，这些化合物显示出几种不同的生物活性，例如大分子和小分子，包括肽、蛋白质/酶和其他组分。因此，多年来，毒液成分吸引了生物化学家和药理学家的注意。棕色寡妇蜘蛛（Latrodectus geometricus）是一种有毒的蜘蛛，在世界各地都有发现，包括泰国。这种蜘蛛会对人类造成伤害，毒液有很多潜在的用途。在这项研究中，我们调查了棕色寡妇蜘蛛毒液的复杂性和药理学。采用部分蛋白质组技术和酶活性、毒性和抗菌活性评估对蜘蛛粗毒进行了研究。我们发现，粗毒液显示了广泛的分子量从19到超过97 kDa，与66 kDa的分子量强烈染色。采用液相色谱-质谱/质谱（LC-MS/MS）联用技术对粗毒中的多肽和蛋白质进行鉴定，结果表明粗毒中含有多种物质，包括latrotoxins、apolipophorins、hemocyanins、chitinases、arginine kinase、allergenantigen 5-like protein、astacin like metalloproteins和serine proteins等。基于比浊法观察到高透明质酸酶活性。该毒液对蟋蟀具有毒性（PD_（50）= 0.73 ± 0.10 μg/g体重），并出现明显的中毒症状，如缓慢运动、麻痹甚至死亡。此外，该毒液对革兰氏阳性枯草芽孢杆菌表现出潜在的抗菌活性，但对革兰氏阴性铜绿假单胞菌没有抗菌活性。蜘蛛毒液含有许多具有生物活性的分子，如影响昆虫的latrotoxins和酶。除了latrotoxins，毒液中的某些酶被假设表现出毒性和抗微生物活性。本研究为进一步开发天然化合物或杀虫毒素提供了重要信息。利用质谱鉴定了8个蛋白质。蛇毒具有潜在的透明质酸酶活性和抗菌活性。天然毒液对蟋蟀有毒性。毒液肽可以开发为天然先导药物或杀虫毒素。
Spiders use their venom for defence and to capture prey. These venoms contain a cocktail of biologically active compounds that display several different biological activities, such as large molecules and small molecules including peptides, proteins/enzymes, and other components. Thus, venom constituents have attracted the attention of biochemists and pharmacologists over the years. The brown widow spider (Latrodectus geometricus) is a venomous spider found worldwide, including in Thailand. This spider causes human injuries, and the venom has many potential applications. In this study, we investigated the complexity and pharmacology of brown widow spider venom. Spider crude venom was investigated using partial proteome techniques and enzymatic activity, toxicity, and antibacterial activity assessments. We found that crude venom displayed a wide range of molecular masses from 19 to over 97 kDa, with molecular masses of 66 kDa intensely stained. Peptides and proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), which showed that the crude venom contained a variety of substances, including latrotoxins, apolipophorins, hemocyanins, chitinases, arginine kinase, allergen antigen 5-like protein, astacin-like metalloproteases, and serine proteases. High hyaluronidase activity was observed based on the turbidimetric method. The venom presented toxicity in crickets (PD50 = 0.73 ± 0.10 μg/g body weight), and substantial envenomation symptoms, such as slow-motion movement, paralysis, and even death, were noted. Moreover, this venom exhibited potential antibacterial activity against the gram-positive Bacillus subtilis but not the gram-negative Pseudomonas aeruginosa. Spider venom contains numerous molecules with biological activity, such as latrotoxins, which affect insects, and enzymes. In addition to latrotoxins, certain enzymes in venom are hypothesized to exhibit toxicity and antimicrobial activity. This study provides important information for the further development of natural compounds or insecticidal toxins. 8 proteins were identified using mass spectrometry. Venom exhibited potential hyaluronidase activity and antibacterial activity. Crude venom presented toxicity in crickets. The venom peptides can develop for a natural lead drug or an insecticidal toxin.
登录
查看更多内容
Lycosin-I 的抗菌潜力，一种来自 Lycosa Singorensis 蜘蛛毒液的阳离子和两亲性肽
影响因子：
2.5
作者：
Tan, H.;Ding, X.;Liang, S.
通讯作者：
Liang, S.
影响因子：
3.8
作者：
Ferrer VP;de Mari TL;Gremski LH;Trevisan Silva D;da Silveira RB;Gremski W;Chaim OM;Senff-Ribeiro A;Nader HB;Veiga SS
通讯作者：
Veiga SS
影响因子：
3.8
作者：
Villas-Boas，Isadora Maria;Goncalves-de-Andrade，Rute Maria;Tambourgi，Denise V.
通讯作者：
Tambourgi，Denise V.
Latrodectus tredecimguttatus 毒腺解剖毒液的提取及蛋白质成分分析
DOI：
10.1016/j.cbpb.2006.08.006
发表时间：
2006-11-01
影响因子：
2.2
作者：
Duan, Z. G.;Yan, X. J.;Liang, S. P.
通讯作者：
Liang, S. P.
影响因子：
1.1
作者：
Goddard, Jerome;Upshaw, Stacy;Johnnson, Kerry
通讯作者：
Johnnson, Kerry