The complete mitochondrial DNA sequence of the pantropical earthworm Pontoscolex corethrurus (Rhinodrilidae, Clitellata): Mitogenome characterization and phylogenetic positioning.

The complete mitochondrial DNA sequence of the pantropical earthworm Pontoscolex corethrurus (Rhinodrilidae, Clitellata): Mitogenome characterization and phylogenetic positioning.
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DOI:
10.3897/zookeys.688.13721
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发表时间:
2017
期刊:
影响因子:
1.3
通讯作者:
Cunha L
Cunha L
中科院分区:
生物学3区
文献类型:
--
作者:
Conrado AC;Arruda H;Stanton DWG;James SW;Peter Kille;Brown G;Silva E;Dupont L;Taheri S;Morgan AJ;Simões N;Rodrigues A;Montiel R;Cunha L

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南美岸蚓(Pontoscolex corethrurus,Müller,1857)在热带土壤生态系统中起着重要作用,并且由于其在受人类干扰的热带土壤中普遍存在且通常数量丰富,已被广泛用作多种生态学研究的动物模型。在本研究中,我们描述了广布种南美岸蚓的完整线粒体基因组。这是在棘蚓科(Rhinodrilidae)内线粒体基因组的首次记录。其线粒体基因组长度为14835bp,包含37个基因(13个蛋白质编码基因、2个rRNA基因和22个tRNA基因)。它具有与其他已测序的蚯蚓相同的基因内容和结构,但在无脊椎动物中不同寻常的是它有几个重叠的开放阅读框。所有基因都在同一条链上编码。大多数蛋白质编码基因使用ATG作为起始密码子,但ND3使用GTG作为起始密码子。线粒体基因组的A + T含量为59.9%(A为31.8%,T为28.1%,G为14.6%,C为25.6%)。注释后的基因组序列已提交至GenBank,登录号为KT988053。
Pontoscolex corethrurus (Müller, 1857) plays an important role in tropical soil ecosystems and has been widely used as an animal model for a large variety of ecological studies in particular due to its common presence and generally high abundance in human-disturbed tropical soils. In this study we describe the complete mitochondrial genome of the peregrine earthworm P. corethrurus. This is the first record of a mitochondrial genome within the Rhinodrilidae family. Its mitochondrial genome is 14 835 bp in length containing 37 genes (13 protein-coding genes (PCG) 2 rRNA genes and 22 tRNA genes). It has the same gene content and structure as in other sequenced earthworms but unusual among invertebrates it hasseveral overlapping open reading frames. All genes are encoded on the same strand. Most of the PCGs use ATG as the start codon except for ND3 which uses GTG as the start codon. The A+T content of the mitochondrial genome is 59.9% (31.8% A 28.1% T 14.6% G and 25.6% for C). The annotated genome sequence has been deposited in GenBank under the accession number KT988053.
DOI: 10.1093/oxfordjournals.molbev.a026011
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