Autogenous Biofabrication of Nativelike, Scaffold-Free Human Skin Equivalents Using a Smart, Enzyme-Degradable Tissue Templating Coating.
Autogenous Biofabrication of Nativelike, Scaffold-Free Human Skin Equivalents Using a Smart, Enzyme-Degradable Tissue Templating Coating.
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使用智能、酶可降解的组织模板涂层自体生物制造类似天然、无支架的人类皮肤等效物。
DOI：
10.1021/acsabm.8b00685
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发表时间：
2019
影响因子：
4.7
通讯作者：
Connon CJ
中科院分区：
文献类型：
--
作者：
Connon CJ
在这项研究中，我们使用组织模板技术指导人类真皮成纤维细胞生物制造大面积组织，密切模仿天然真皮。该技术还允许新组织促进其自身从模板表面释放，从而促进其作为仅包含人细胞和其自身细胞外基质的自我维持的无支架真皮等同物的恢复。这些真皮自提升自体组织等效物（SLATE）的结构和组成进行了详细评估，并显示与正常组织功能密切相关。具体而言，真皮SLATE由致密的胶原基基质与真皮特征性弹性纤维交织组成。此外，这些组织的机械性能（即，坚固性、弹性模量和抗收缩性和酶降解性）与天然人真皮的那些相当。此外，皮肤SLATE能够构成组织作为一个基板，以支持角质形成细胞的生长成分层上皮细胞分化的不同层的高阶复杂性。因此，这项工作说明了组织模板技术的巨大潜力，以及这些技术如何为易于回收的无支架人体皮肤组织的生物制造铺平道路，该组织具有适合临床和非临床应用的结构，组成和功能。
In this study, we used tissue templating technology to direct human dermal fibroblasts to biofabricate large-area tissues that closely emulate the natural dermis. This technology also allowed the new tissues to promote their own release from the template surface, thus facilitating their recovery as self-sustained, scaffold-free dermal equivalents solely comprising human cells and their own extracellular matrix. The structure and composition of these dermal self-lifting autogenous tissue equivalents (SLATEs) were evaluated in detail and were shown to closely correlate to normal tissue function. Specifically, dermal SLATEs were shown to be composed of a dense collagen-based matrix interwoven with dermal-characteristic elastic fibers. In addition, the mechanical properties of these tissues (i.e., robustness, elastic modulus, and resistance to contraction and enzymatic degradation) were comparable to those of the natural human dermis. Furthermore, dermal SLATEs were capable of constituting tissues with a higher-order complexity by serving as a substrate to support the growth of keratinocytes into stratified epithelia with distinct layers of differentiation. This work thus illustrates the great potential of tissue templating technologies and how these can pave the way for the biofabrication of easily retrievable, scaffold-free human skin tissues with a structure, composition, and function suitable for both clinical and nonclinical applications.
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影响因子：
4.9
作者：
J. R. Sharpe;Y. Martin
通讯作者：
Y. Martin
影响因子：
9.7
作者：
Grant, C. A.;Twigg, P. C.;Tobin, D. J.
通讯作者：
Tobin, D. J.
弹性蛋白在伤口愈合和真皮替代品设计中的作用
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
J. Rnjak‐Kovacina;A. Weiss
通讯作者：
A. Weiss
影响因子：
17.1
作者：
Fang, Ming;Goldstein, Elizabeth L.;Turner, A. Simon;Les, Clifford M.;Orr, Bradford G.;Fisher, Gary J.;Welch, Kathleen B.;Rothman, Edward D.;Holl, Mark M. Banaszak
通讯作者：
Holl, Mark M. Banaszak
影响因子：
4.8
作者：
Bielefeld, Kirsten A.;Amini-Nik, Saeid;Alman, Benjamin A.
通讯作者：
Alman, Benjamin A.