Ultrasensitive determination of DNA sequences by flow injection chemiluminescence using silver ions as labels.
Ultrasensitive determination of DNA sequences by flow injection chemiluminescence using silver ions as labels.
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使用银离子作为标记，通过流动注射化学发光法超灵敏测定 DNA 序列
DOI：
10.1016/j.aca.2014.07.033
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发表时间：
2014-10
影响因子：
6.2
通讯作者：
Lu, Xiaoquan
中科院分区：
文献类型：
--
作者：
Zhou, Min;Ma, Yongjun;Wu, Guofan;Lu, Xiaoquan
本文提出了一种以金属硫蛋白（MT）为桥梁，以Ag+标记的新型检测探针为基础的DNA序列超灵敏检测新策略。该检测依赖于三明治式DNA杂交，首先将DNA靶标与固定在Fe3O4@Au复合磁性纳米颗粒（MNPs）上的捕获的寡核苷酸探针杂交，然后使用Ag+修饰的检测探针监测特定DNA靶标的存在。Ag+被锚定在杂化物上后，通过酸性处理向下释放，并通过流动注射-化学发光反应体系（Ag+ -Mn2 + -K2S2O8-H3PO4-luminol） （FI-CL）进行敏感测定。实验结果表明，在10 ~ 500 pmol L−1范围内，检测限为3.3 pmol L−1，荧光强度随靶DNA浓度的增加呈线性增加。本研究的高灵敏度可能归因于Ag+ -MT的高摩尔比、FI-CL偶联反应体系对Ag+的灵敏测定以及Fe3O4@Au复合MNPs的完美磁分离。此外，该策略对错配DNA序列具有良好的选择性，可应用于实际样品分析。
We presented a new strategy for ultrasensitive detection of DNA sequences based on the novel detection probe which was labeled with Ag+using metallothionein (MT) as a bridge. The assay relied on a sandwich-type DNA hybridization in which the DNA targets were first hybridized to the captured oligonucleotide probes immobilized on Fe3O4@Au composite magnetic nanoparticles (MNPs), and then the Ag+-modified detection probes were used to monitor the presence of the specific DNA targets. After being anchored on the hybrids, Ag+was released down through acidic treatment and sensitively determined by a coupling flow injection–chemiluminescent reaction system (Ag+–Mn2+–K2S2O8–H3PO4–luminol) (FI–CL). The experiment results showed that the CL intensities increased linearly with the concentrations of DNA targets in the range from 10 to 500 pmol L−1with a detection limit of 3.3 pmol L−1. The high sensitivity in this work may be ascribed to the high molar ratio of Ag+–MT, the sensitive determination of Ag+by the coupling FI–CL reaction system and the perfect magnetic separation based on Fe3O4@Au composite MNPs. Moreover, the proposed strategy exhibited excellent selectivity against the mismatched DNA sequences and could be applied to real samples analysis.
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影响因子：
3.3
作者：
MANSFIELD, ES;WORLEY, JM;FORTINA, P
通讯作者：
FORTINA, P
DOI：
10.1002/chin.200805278
发表时间：
2008-01
期刊：
ChemInform
影响因子：
--
作者：
K. Klepárník;P. Boček
通讯作者：
K. Klepárník;P. Boček
影响因子：
7.4
作者：
Youyu Zhang;Zhiwen Tang;Jun Wang;Hong Wu;A. Maham;Yuehe Lin
通讯作者：
Youyu Zhang;Zhiwen Tang;Jun Wang;Hong Wu;A. Maham;Yuehe Lin
影响因子：
2.5
作者：
Tamer, Ugur;Gundogdu, Yusuf;Pekmez, Kadir
通讯作者：
Pekmez, Kadir
影响因子：
4.6
作者：
A. Żelazowski;M. Stillman
通讯作者：
A. Żelazowski;M. Stillman