Quantifying male and female pheromone-based mate choice in Caenorhabditis nematodes using a novel microfluidic technique.

Quantifying male and female pheromone-based mate choice in Caenorhabditis nematodes using a novel microfluidic technique.
复制标题

DOI:
10.1371/journal.pone.0189679
复制
发表时间:
2017
期刊:
影响因子:
3.7
通讯作者:
Phillips PC
Phillips PC
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Borne F;Kasimatis KR;Phillips PC

文献摘要

参考文献

被引文献

相似文献

信息素线索是两性沟通的重要组成部分,特别是在配偶选择方面。小杆线虫主要依靠产生信息素来寻找配偶和选择配偶。在这里,我们描述了一个新的微流体模式研究线虫的配偶选择。具体来说,信息素竞技场允许气味的恒定流动,包括信息素和其他小分子,在真实的时间内从信号蠕虫传递到那些做出选择的蠕虫,而无需任何身体接触。我们通过证实以前的研究证实了这种微流体范例,表明原始C。remanei和C.秀丽线虫雄性对处女雌性的偏好超过交配的雌性。此外,我们的研究结果表明,吸引力的强度是一个加性效应的男性接受和女性的信号产生。我们还明确检查女性的选择,发现女性更吸引处女男性。然而,雌性的择偶强烈地依赖于她的交配状态。
Pheromone cues are an important component of intersexual communication, particularly in regards to mate choice. Caenorhabditis nematodes predominant rely on pheromone production for mate finding and mate choice. Here we describe a new microfluidic paradigm for studying mate choice in nematodes. Specifically, the Pheromone Arena allows for a constant flow of odorants, including pheromones and other small molecules, to be passed in real time from signaling worms to those making a choice without any physical contact. We validated this microfluidic paradigm by corroborating previous studies in showing that virgin C. remanei and C. elegans males have a strong preference for virgin females over mated ones. Moreover, our results suggest that the strength of attraction is an additive effect of male receptivity and female signal production. We also explicitly examine female choice and find that females are more attracted to virgin males. However, a female’s mate choice is strongly dependent on her mating status.
DOI: 10.1523/jneurosci.1746-04.2004
发表时间: 2004-08-25
影响因子: 5.3
作者:
Lipton, J;Kleemann, G;Emmons, SW
通讯作者: Emmons, SW
DOI: 10.3390/mi7070123
发表时间: 2016-07-19
期刊: Micromachines
影响因子: 3.4
作者:
Gupta BP;Rezai P
通讯作者: Rezai P
DOI: 10.1039/c1lc20400a
发表时间: 2011-11-07
期刊: Lab on a chip
影响因子: 6.1
作者:
Chung K;Zhan M;Srinivasan J;Sternberg PW;Gong E;Schroeder FC;Lu H
通讯作者: Lu H
DOI: 10.1098/rspb.2005.3332
发表时间: 2006-02-07
影响因子: 4.7
作者:
Grillet, M;Dartevelle, L;Ferveur, JF
通讯作者: Ferveur, JF
DOI: 10.1534/genetics.106.068304
发表时间: 2007-04-01
期刊: GENETICS
影响因子: 3.3
作者:
Garcia, L. Rene;LeBoeuf, Brigitte;Koo, Pamela
通讯作者: Koo, Pamela