Microfluidic chamber arrays for whole-organism behavior-based chemical screening.
Microfluidic chamber arrays for whole-organism behavior-based chemical screening.
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DOI：
10.1039/c1lc20400a
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发表时间：
2011-11-07
期刊：
影响因子：
6.1
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Chung K;Zhan M;Srinivasan J;Sternberg PW;Gong E;Schroeder FC;Lu H
秀丽隐杆线虫是遗传学研究和药物筛选的重要模式生物，因为它相对简单，易于维护，易于简单的遗传操作，并与人类生物学相关。然而，它们的小尺寸和移动性使得线虫难以物理操纵，特别是空间和时间精度。我们已经开发了一种微流控设备来克服这些挑战，并实现C中基于行为的快速化学筛选。优雅的这种易于使用的设备的关键组件允许快速加载和住房的C。在室阵列中进行化学筛选。一个简单的两步装载过程可以在几分钟内同时装载大量动物，而无需使用任何昂贵/有源的片外组件。此外，化学品可以精确地输送到蠕虫，并以高时间精度进行交换。为了证明这一功能和测量时间依赖性的化学反应的能力，我们表征了蠕虫暴露于不同浓度的麻醉剂的瞬态反应。然后，我们使用该设备来研究雌雄同体蠕虫的化学信号对雄性行为的影响。该装置在一个视野中长时间保持大量自由移动动物的能力使我们能够证明在接受蠕虫条件培养基的雄性中特定行为的发生率增加。因为我们的设备允许监测大量的蠕虫与单个动物的分辨率，我们设想，这个平台将大大加快化学筛选在C。优美的
The nematode Caenorhabditis elegans is an important model organism in genetic research and drug screening because of its relative simplicity, ease of maintenance, amenability to simple genetic manipulation, and relevance to human biology. However, their small size and mobility make nematodes difficult to physically manipulate, particularly with spatial and temporal precision. We have developed a microfluidic device to overcome these challenges and enable fast behavior-based chemical screening in C. elegans. The key components of this easy-to-use device allow rapid loading and housing of C. elegans in a chamber array for chemical screening. A simple two-step loading process enables simultaneous loading of a large number of animals within a few minutes without using any expensive/active off-chip components. In addition, chemicals can be precisely delivered to the worms and exchanged with high temporal precision. To demonstrate this feature and the ability to measure time dependent responses to chemicals, we characterize the transient response of worms exposed to different concentrations of anesthetics. We then use the device to study the effect of chemical signals from hermaphrodite worms on male behavior. The ability of the device to maintain a large number of free moving animals in one field of view over a long period of time permits us to demonstrate an increase in the incidence of a specific behavior in males subjected to worm-conditioned medium. Because our device allows monitoring of a large number of worms with single-animal resolution, we envision that this platform will greatly expedite chemical screening in C. elegans.
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影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
5.3
作者：
Miller, AC;Thiele, TR;Lockery, SR
通讯作者：
Lockery, SR
影响因子：
16.2
作者：
McGrath, Patrick T.;Rockman, Matthew V.;Zimmer, Manuel;Jang, Heeun;Macosko, Evan Z.;Kruglyak, Leonid;Bargmann, Cornelia I.
通讯作者：
Bargmann, Cornelia I.
影响因子：
5.3
作者：
Iino, Yuichi;Yoshida, Kazushi
通讯作者：
Yoshida, Kazushi
影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC