Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis.
Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis.
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DOI：
10.1021/pr200918f
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发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Xia, Yiji
中科院分区：
文献类型：
--
作者：
Wang, Hai;Wang, Shengbing;Lu, Yuqing;Avarez, Sophie;Hicks, Leslie M.;Ge, Xiaochun;Xia, Yiji
通过氧化修饰调节蛋白质功能已成为调节各种生物过程的重要分子机制。在这里，我们报告的蛋白质组学研究氧化还原敏感蛋白在拟南芥细胞受到过氧化氢处理。四个基于凝胶的方法，导致四个部分重叠的蛋白质组的鉴定，其巯基在过氧化氢处理的细胞中进行氧化修饰。使用的方法的基础上的差异标记的巯基，然后通过免疫沉淀和蛋白质印迹法，5的6个选定的假定氧化还原敏感蛋白被证实进行氧化修饰后的氧化剂处理在拟南芥叶片。另一种方法，这是基于差异标记的巯基结合蛋白电泳迁移率变动分析，通过揭示H2O2敏感的蛋白，一个同源的甜菜碱诱导的凋亡抑制因子1（AtCIAPIN 1），也经历了氧化修饰后，在拟南芥叶片中的水杨酸或肽激发子flg22，两个诱导剂的防御信号。从蛋白质组学研究中鉴定的氧化还原敏感蛋白参与各种生物过程，如代谢、抗氧化系统、蛋白质生物合成和加工以及细胞骨架组织。新的氧化还原敏感蛋白的鉴定将有助于了解以前未知的氧化还原调节的细胞组分或途径。通过四个氧化还原蛋白质组学方法，我们确定了一些拟南芥蛋白质，经历了快速氧化修饰的拟南芥细胞后过氧化氢处理。我们还建立了两种方法来详细分析个别推定的氧化还原敏感蛋白。氧化剂敏感蛋白的鉴定将非常有助于深入表征以前未知的氧化还原调节的其他信号通路。
Regulation of protein function through oxidative modification has emerged as an important molecular mechanism modulating various biological processes. Here, we report a proteomic study of redox-sensitive proteins in Arabidopsis cells subjected to H2O2 treatment. Four gel-based approaches were employed, leading to the identification of four partially overlapping sets of proteins whose thiols underwent oxidative modification in the H2O2-treated cells. Using a method based on differential labeling of thiols followed by immunoprecipitation and Western blotting, five of the six selected putative redox-sensitive proteins were confirmed to undergo oxidative modification following the oxidant treatment in Arabidopsis leaves. Another method, which is based on differential labeling of thiols coupled with protein electrophoretic mobility shift assay, was adopted to reveal that one of the H2O2-sensitive proteins, a homologue of cytokine-induced apoptosis inhibitor 1 (AtCIAPIN1), also underwent oxidative modification in Arabidopsis leaves after treatments with salicylic acid or the peptide elicitor flg22, two inducers of defense signaling. The redox-sensitive proteins identified from the proteomic study are involved in various biological processes such as metabolism, the antioxidant system, protein biosynthesis and processing, and cytoskeleton organization. The identification of novel redox-sensitive proteins will be helpful toward understanding of cellular components or pathways previously unknown to be redox-regulated. Through four redox proteomic methods, we identified a number of Arabidopsis proteins that underwent rapid oxidative modifications in Arabidopsis cells upon H2O2 treatment. We also established two methods for detailed analysis of individual putative redox-sensitive proteins. The identification of the oxidant-sensitive proteins would be greatly helpful toward in-depth characterization of other signaling pathways previously unknown to be redox-regulated.
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影响因子：
64.8
作者：
Delledonne, M;Xia, YJ;Lamb, C
通讯作者：
Lamb, C
DOI：
10.1073/pnas.95.17.10328
发表时间：
1998-08-18
影响因子：
11.1
作者：
Durner, J;Wendehenne, D;Klessig, DF
通讯作者：
Klessig, DF
影响因子：
7.2
作者：
Baier, M;Dietz, KJ
通讯作者：
Dietz, KJ
影响因子：
7.4
作者：
Galletti, Roberta;Denoux, Carine;Ferrari, Simone
通讯作者：
Ferrari, Simone
影响因子：
6.5
作者：
Hancock, JT;Henson, D;Neill, SJ
通讯作者：
Neill, SJ