Radiotherapy effect on nano-mechanical properties and chemical composition of enamel and dentine.
Radiotherapy effect on nano-mechanical properties and chemical composition of enamel and dentine.
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DOI：
10.1016/j.archoralbio.2015.02.020
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发表时间：
2015-05
影响因子：
3
通讯作者：
Walker, M. P.
中科院分区：
文献类型：
--
作者：
Reed, R.;Xu, C.;Liu, Y.;Gorski, J. P.;Wang, Y.;Walker, M. P.
为了了解放射治疗引起的牙损害，其特征在于牙本质-牙釉质交界处（DEJ）附近的釉质损失或分层，本研究评估了模拟口腔癌放射治疗前后牙釉质和牙本质的纳米机械性能和化学成分。将7个非龋性第三磨牙的切片暴露于2戈伊剂量，每周5天，持续7周，总计70戈伊。纳米压痕被用来评估杨氏模量，而拉曼显微光谱被用来测量蛋白质/矿物质的比例，碳酸盐/磷酸盐的比例，和磷酸盐的峰宽。所有测量均在照射前后在釉质和牙本质中距DEJ 500和30微米的相同四个颊侧和舌侧部位（E-500、E-30、D-30和D-500）完成。照射后牙釉质和牙本质的弹性模量均显著增加（P≤0.05）。根据拉曼光谱分析，除了D-500以外，在所有试验地点，辐射后蛋白质与矿物质的比率（2931/430 cm-1）显著降低，而碳酸盐与磷酸盐的比率（1070/960 cm-1）在E-30增加，在D-500减少。最后，在960 cm-1处通过FWHM测量的磷酸盐峰宽度在辐射后的D-30和D-500处均显著降低。模拟放射治疗产生的增加，在刚度的牙釉质和牙本质附近的DEJ。据推测，硬度增加是辐射诱导蛋白质含量减少的结果，其中釉质部位的减少百分比要大得多。机械性能和化学成分的这种变化可能会导致DEJ生物力学失效，导致放疗后发生的釉质脱层。然而，为了更好地了解放射治疗对牙齿结构的影响，以改善口腔癌患者的预防和恢复治疗，还需要进行其他分析。
To understand radiotherapy-induced dental lesions characterized by enamel loss or delamination near the dentin-enamel junction (DEJ), this study evaluated enamel and dentin nano-mechanical properties and chemical composition before and after simulated oral cancer radiotherapy. Sections from seven non-carious third molars were exposed to 2 Gy fractions, 5 days/week for 7 weeks for a total of 70 Gy. Nanoindentation was used to evaluate Young’s modulus, while Raman microspectroscopy was used to measure protein/mineral ratios, carbonate/phosphate ratios, and phosphate peak width. All measures were completed prior to and following radiation at the same four buccal and lingual sites 500 and 30 microns from the DEJ in enamel and dentin (E-500, E-30, D-30 and D-500). The elastic modulus of enamel and dentin was significantly increased (P≤0.05) following radiation. Based on Raman spectroscopic analysis, there was a significant decrease in the protein to mineral ratio (2931/430 cm-1) following radiation at all sites tested except at D-500, while the carbonate to phosphate ratio (1070/960 cm-1) increased at E-30 and decreased at D-500. Finally, phosphate peak width as measured by FWHM at 960 cm-1 significantly decreased at both D-30 and D-500 following radiation. Simulated radiotherapy produced an increase in the stiffness of enamel and dentin near the DEJ. Increased stiffness is speculated to be the result of the radiation-induced decrease in the protein content, with the percent reduction much greater in the enamel sites. Such changes in mechanical properties and chemical composition could potentially contribute to DEJ biomechanical failure leading to enamel delamination that occurs post-radiotherapy. However, other analyses are required for a better understanding of radiotherapy-induced effects on tooth structure to improve preventive and restorative treatments for oral cancer patients.
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影响因子：
2.4
作者：
Habelitz, S;Marshall, GW;Marshall, SJ
通讯作者：
Marshall, SJ
影响因子：
5
作者：
Hobson, RS;McCabe, JF;Hogg, SD
通讯作者：
Hogg, SD
影响因子：
5.7
作者：
LESLIE, MD;DISCHE, S
通讯作者：
DISCHE, S
DOI：
10.1016/s0300-9785(85)80038-7
发表时间：
1985-01-01
期刊：
INTERNATIONAL JOURNAL OF ORAL SURGERY
影响因子：
--
作者：
ANNEROTH, G;HOLM, LE;KARLSSON, G
通讯作者：
KARLSSON, G
影响因子：
4.4
作者：
McGuire JD;Mousa AA;Zhang BJ;Todoki LS;Huffman NT;Chandrababu KB;Moradian-Oldak J;Keightley A;Wang Y;Walker MP;Gorski JP
通讯作者：
Gorski JP