Extracts of irradiated mature human tooth crowns contain MMP-20 protein and activity.
Extracts of irradiated mature human tooth crowns contain MMP-20 protein and activity.
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DOI：
10.1016/j.jdent.2014.02.013
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发表时间：
2014-05
影响因子：
4.4
通讯作者：
Gorski JP
中科院分区：
文献类型：
--
作者：
McGuire JD;Mousa AA;Zhang BJ;Todoki LS;Huffman NT;Chandrababu KB;Moradian-Oldak J;Keightley A;Wang Y;Walker MP;Gorski JP
我们最近证明了口腔癌患者的牙釉质分层与牙齿水平辐射剂量之间存在显着相关性。由于辐射可以诱导基质金属蛋白酶的合成和活化，因此我们假设受辐射的牙齿可能含有活性基质金属蛋白酶。对接受放射治疗的口腔癌患者和健康受试者拔出的牙齿进行了比较。对健康受试者拔出的成熟第三磨牙进行体外照射和/或在 37°C 下孵育 0 至 6 个月。然后将所有牙齿粉碎、提取，并对提取物进行蛋白质组和酶分析。使用质谱法对辐照牙冠提取物进行筛选，鉴定出 MMP-20（釉质溶解素），它在牙本质和牙釉质中发育表达，但据信在牙齿萌出之前被去除。 MMP-20 由 Mr=43、41、24 和 22 kDa 的催化活性形式组成，主要免疫定位于形态学牙本质釉质连接处。不同大小的MMP-20形式的比例随着孵育和照射而变化。虽然用 70 G 照射健康牙齿不会直接改变模式，但随后在 37°C 下孵育 3-6 个月（无论是否事先照射）都会导致 Mr=24-22 kDa MMP-20 带的比例急剧增加。接受 >70 Gy 辐射的口腔癌患者的牙齿提取物也比健康的年龄相关牙齿含有相对更多的 24 和 22 kDa MMP-20。 MMP-20 是成熟牙冠的抗辐射成分，富含牙本质釉质。我们推测 MMP-20 催化该位点有机基质的降解可能导致与口腔癌放射治疗相关的牙釉质分层。
We recently demonstrated a significant correlation between enamel delamination and tooth-level radiation dose in oral cancer patients. Since radiation can induce the synthesis and activation of matrix metalloproteinases, we hypothesized that irradiated teeth may contain active matrix metalloproteinases. Extracted teeth from oral cancer patients treated with radiotherapy and from healthy subjects were compared. Extracted mature third molars from healthy subjects were irradiated in vitro and/or incubated for 0 to 6 months at 37°C. All teeth were then pulverized, extracted, and extracts subjected to proteomic and enzymatic analyses. Screening of irradiated crown extracts using mass spectrometry identified MMP-20 (enamelysin) which is expressed developmentally in dentin and enamel but believed to be removed prior to tooth eruption. MMP-20 was composed of catalytically active forms at Mr=43, 41, 24 and 22 kDa and was immunolocalized predominantly to the morphological dentin enamel junction. The proportion of different sized MMP-20 forms changed with incubation and irradiation. While the pattern was not altered directly by irradiation of healthy teeth with 70 G, subsequent incubation at 37°C for 3–6 months with or without prior irradiation caused the proportion of Mr=24–22 kDa MMP-20 bands to increase dramatically. Extracts of teeth from oral cancer patients who received >70 Gy radiation also contained relatively more 24 and 22 kDa MMP-20 than those of healthy age-related teeth. MMP-20 is a radiation-resistant component of mature tooth crowns enriched in the dentin-enamel. We speculate that MMP-20 catalyzed degradation of organic matrix at this site could lead to enamel delamination associated with oral cancer radiotherapy.
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影响因子：
1.9
作者：
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通讯作者：
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影响因子：
7.6
作者：
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作者：
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通讯作者：
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作者：
Strup-Perrot, C;Vozenin-Brotons, MC;Mathe, D
通讯作者：
Mathe, D