Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.
Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.
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DOI：
10.1155/2017/2905104
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发表时间：
2017
影响因子：
4.3
通讯作者：
El Haj AJ
中科院分区：
文献类型：
--
作者：
Chapman V;Markides H;Sagar DR;Xu L;Burston JJ;Mapp P;Kay A;Morris RH;Kehoe O;El Haj AJ
间充质干细胞 (MSC) 具有治疗骨关节炎 (OA) 关节病理和疼痛的潜力。本研究的目的是确定传代次数对间充质干细胞对 OA 啮齿动物模型疼痛行为以及软骨和骨特征的影响的影响。 大鼠在麻醉下接受内侧半月板横断术（MNX）或假手术。大鼠关节内注射1.5 × 106个用10μg/ml SiMAG标记的晚期传代间充质干细胞、1.5 × 106个晚期传代间充质干细胞、类固醇Kenalog (200 μg/20 μL)、1.5 × 106个早期传代间充质干细胞或无血清培养基（可持续森林管理）。假手术大鼠接受关节内注射SFM。直到模型诱导后第 42 天，疼痛行为均被量化。磁共振成像（MRI）用于定位膝关节内的标记细胞。 晚期传代的 MSC 和 Kenalog 减轻了 MNX 大鼠的既定疼痛行为，但在研究结束时并没有改变 MNX 诱导的关节病理学。早期传代的 MSC 会在注射后长达一周的时间内加剧 MNX 诱导的疼痛行为，并且不会改变关节病理学。 我们的数据首次证明了传代次数对 OA 疼痛模型中 MSC 治疗效果的影响。
Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA. Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5 × 106 late passage MSCs labelled with 10 μg/ml SiMAG, 1.5 × 106 late passage mesenchymal stem cells, the steroid Kenalog (200 μg/20 μL), 1.5 × 106 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint. Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology. Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.
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影响因子：
7
作者：
Malfait, A. M.;Little, C. B.;McDougall, J. J.
通讯作者：
McDougall, J. J.
影响因子：
7.5
作者：
Markides H;Kehoe O;Morris RH;El Haj AJ
通讯作者：
El Haj AJ
骨关节炎动物模型中关节内注射自体间充质干细胞的归巢和修复作用。
影响因子：
2.3
作者：
Mokbel AN;El Tookhy OS;Shamaa AA;Rashed LA;Sabry D;El Sayed AM
通讯作者：
El Sayed AM
影响因子：
3.7
作者：
Burston JJ;Sagar DR;Shao P;Bai M;King E;Brailsford L;Turner JM;Hathway GJ;Bennett AJ;Walsh DA;Kendall DA;Lichtman A;Chapman V
通讯作者：
Chapman V
影响因子：
7
作者：
Mapp, P. I.;Sagar, D. R.;Ashraf, S.;Burston, J. J.;Suri, S.;Chapman, V.;Walsh, D. A.
通讯作者：
Walsh, D. A.