Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model.
Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model.
复制标题
骨关节炎动物模型中关节内注射自体间充质干细胞的归巢和修复作用。
DOI：
10.1186/1471-2474-12-259
复制
发表时间：
2011-11-15
影响因子：
2.3
通讯作者：
El Sayed AM
中科院分区：
文献类型：
--
作者：
Mokbel AN;El Tookhy OS;Shamaa AA;Rashed LA;Sabry D;El Sayed AM
本研究旨在通过实验动物模型（驴）研究骨髓间充质干细胞（MSCs）在诱导性骨关节炎愈合过程中的归巢证据和修复作用。将27头驴按诱导关节炎后观察期（3、6、9周）分为3组，每组27头，以达到不同程度的骨关节炎。根据给药后的随访期（1、2和6个月），将每组再分为3个亚组，每组3只动物。通过在两个腕关节关节内（IA）注射2 ml两性霉素B进行诱导。在单独的程序中收获MSC，使用monster GFP载体用绿色荧光蛋白（GFP）标记，并悬浮在透明质酸中用于IA注射。治疗方法包括使用悬浮在3 ml透明质酸（HA）中的MSC对右侧腕关节进行细胞治疗;以及使用相同量的（HA）但不含MSC对左侧对侧腕关节进行细胞治疗作为对照。在给药前后对动物进行临床和放射学评估。还评价了滑液。组织学上;对关节软骨结构变化、关节软骨基质染色减少、骨赘形成和软骨下骨板增厚进行分级。使用组织病理学分级评分的中位数和百分位数总结数据。使用非参数Mann Whitney检验进行组间比较。与对照组相比，各治疗组的临床和影像学修复效果均显著（P < 0.05）。所有动物的细胞处理关节的切片的荧光显微镜检查表明，GFP转导的注射细胞有效地参与了受损关节表面的修复过程，并整合在现有的关节软骨内。这些细胞与软骨的表面相关联，并且在内部也被检测到。通过将注射的GFP标记的MSC掺入修复的新形成的软骨内来确认归巢。显著的恢复证明使用IA注射自体MSC是治疗不同程度骨关节炎的可行且实用的选择。
This work aimed to study the homing evidence and the reparative effect of mesenchymal stem cells (MSCs) in the healing process of induced osteoarthritis in experimental animal model (donkeys). Twenty-seven donkeys were equally divided into 3 groups based on the observation period after induction of arthritis (3, 6 and 9 weeks) to achieve different degrees of osteoarthritis. Each group was subdivided into three subgroups of three animals each based on the follow-up period (1, 2 and 6 months) after treatment. The induction was done through intra-articular (IA) injection of 2 ml of Amphotericin-B in both carpal joints. MSCs were harvested in a separate procedure, labeled with green fluorescent protein (GFP) using monster GFP vector and suspended in hyaluronic acid for IA injection. Treatment approaches consisted of cell-treatment using MSCs suspended in 3 ml of hyaluronic acid (HA) for the right carpal joint; and using the same amount of (HA) but without MSCs for the left contralateral carpal joint to serve as a control. Animals were assessed clinically and radiologically before and after treatment. Synovial fluid was also evaluated. Histopathologically; articular cartilage structural changes, reduction of articular cartilage matrix staining, osteophyte formation, and subchondral bone plate thickening were graded. Data was summarized using median and percentile for scores of histopathologic grading. Comparison between groups was done using non-parametric Mann Whitney test. The reparative effect of MSCs was significant both clinically and radiologically in all treated groups (P < 0.05) compared to the control groups. Fluorescence microscopy of sections of the cell-treated joints of all animals indicated that the GFP-transduced injected cells have participated effectively in the reparative process of the damaged articular surface and have integrated within the existing articular cartilage. The cells were associated with the surface of the cartilage and, were also detected in the interior. Homing was confirmed by the incorporation of injected GFP-labeled MSCs within the repaired newly formed cartilage. Significant recovery proves that the use of IA injection of autologous MSCs is a viable and a practical option for treating different degrees of osteoarthritis.
登录
查看更多内容
影响因子：
--
作者：
Kollar K;Cook MM;Atkinson K;Brooke G
通讯作者：
Brooke G
影响因子：
4
作者：
Caplan, Arnold I.;Dennis, James E.
通讯作者：
Dennis, James E.
DOI：
10.1111/j.1439-0442.2006.00837.x
发表时间：
2006-08-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE
影响因子：
--
作者：
Marttinen, P. H.;Raulo, S. M.;Tulamo, R. -M.
通讯作者：
Tulamo, R. -M.
影响因子：
7
作者：
Guidolin, DD;Ronchetti, IP;Frizziero, L
通讯作者：
Frizziero, L
影响因子：
8.8
作者：
Hu, Sheng-Li;Luo, Hai-Shui;Feng, Hua
通讯作者：
Feng, Hua