CEACAM6 as detected by the AP11 antibody is a marker notable for mucin-producing adenocarcinomas.
CEACAM6 as detected by the AP11 antibody is a marker notable for mucin-producing adenocarcinomas.
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DOI：
10.1007/s00428-014-1688-1
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发表时间：
2015-02
期刊：
影响因子：
3.5
通讯作者：
Song, Hyung Geun
中科院分区：
文献类型：
--
作者：
Lee, Ok-Jun;Son, Seung-Myoung;Hong, Kwon Pyo;Lee, Yong-Moon;Kim, Min-Young;Choi, Jae-Woon;Lee, Sang-Jeon;Song, Young-Jin;Kim, Hak Soon;Kim, Wun-Jae;Shin, See-Ok;Song, Hyung Geun
用植物血凝素激活的人外周血单个核细胞免疫BALB/c小鼠，获得了一株识别CEACAM 6的单克隆抗体，命名为AP 11。本研究旨在评估CEACAM 6是否可以作为使用AP 11的肿瘤标志物。我们采用流式细胞术检测了11种人癌细胞系中CEACAM 6和AP 11的表达，并采用免疫组化方法检测了439例人组织（包括282例肿瘤组织和157例正常组织）中CEACAM 6和AP 11的表达。AP 11识别的CEACAM 6表位在福尔马林固定和石蜡包埋的组织中保存良好。胃癌（86%）、结直肠癌（95%）、胰腺癌（100%）和肺癌（83%）、膀胱癌（100%）和粘液性卵巢肿瘤（88%）的CEACAM 6免疫反应性比率较高。我们观察到CEACAM 6在肝细胞癌（35%）、肺鳞状细胞癌（60%）、肾细胞癌（14%）、尿路上皮癌（13%）、卵巢浆液性癌（17%）和乳腺癌（11%）中的可变表达。肺小细胞癌、前列腺癌、甲状腺乳头状癌、恶性黑色素瘤、巨细胞瘤和骨肉瘤对CEACAM 6呈阴性。各器官的所有正常组织均为CEACAM 6阴性。总之，由AP 11检测到的CEACAM 6可以作为胃肠道和卵巢的产生粘蛋白的腺癌以及非小细胞肺癌的标志物。因此，AP 11代表了检测CEACMA 6阳性癌症的有价值的诊断工具。
A new monoclonal antibody recognizing CEACAM6, which we named AP11, was generated by immunizing BALB/c mice with phytohemagglutinin-activated human peripheral blood mononuclear cells. This study aims to evaluate whether CEACAM6 can serve as a tumor marker using AP11. We examined the expression of CEACAM6 with AP11 in 11 human carcinoma cell lines by flow cytometry and 439 human tissues including 282 tumor tissues and 157 normal tissues by immunohistochemistry. CEACAM6 epitope recognized by AP11 was well preserved in formalin-fixed and paraffin-embedded tissues. Adenocarcinomas of the stomach (86 %), colorectum (95 %), pancreas (100 %), and lung (83 %), urinary bladder (100 %), and mucinous ovarian tumors (88 %) had a high rate of CEACAM6 immunoreactivity. We observed a variable expression of CEACAM6 in hepatocellular carcinomas (35 %), squamous cell carcinomas of the lung (60 %), renal cell carcinomas (14 %), urothelial carcinomas (13 %), serous carcinomas of the ovary (17 %), and breast carcinomas (11 %). Small-cell carcinomas of the lung, prostatic adenocarcinomas, papillary thyroid carcinomas, malignant melanomas, giant cell tumors, and osteosarcomas were negative for CEACAM6. All normal tissues of various organs were negative for CEACAM6. In conclusion, CEACAM6 as detected by AP11, may serve as a marker for mucin-producing adenocarcinomas of the gastrointestinal tract and ovary as well as non-small cell lung cancer. Thus, AP11 represents a valuable diagnostic tool for detecting CEACMA6-positive cancers.
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影响因子：
8
作者：
Duxbury, MS;Ito, H;Whang, EE
通讯作者：
Whang, EE
影响因子：
8.4
作者：
Lewis-Wambi, Joan S.;Cunliffe, Heather E.;Kim, Helen R.;Willis, Amanda L.;Jordan, V. Craig
通讯作者：
Jordan, V. Craig
影响因子：
2
作者：
Lorenzi, M;Vindigni, C;Francini, G
通讯作者：
Francini, G
影响因子：
11.2
作者：
Duxbury, MS;Ito, H;Whang, EE
通讯作者：
Whang, EE
DOI：
10.1084/jem.122.3.467
发表时间：
1965-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gold P;Freedman SO
通讯作者：
Freedman SO