Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization.
Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization.
复制标题
DOI：
10.1016/j.chom.2013.07.006
复制
发表时间：
2013-08-14
影响因子：
30.3
通讯作者：
McFall-Ngai MJ
中科院分区：
文献类型：
--
作者：
Kremer N;Philipp EE;Carpentier MC;Brennan CA;Kraemer L;Altura MA;Augustin R;Häsler R;Heath-Heckman EA;Peyer SM;Schwartzman J;Rader BA;Ruby EG;Rosenstiel P;McFall-Ngai MJ
在转运到定植位点后，细菌经常经历关键的启动，使其为随后与宿主的特异性相互作用做好准备;然而，基本机制描述不多。在细菌费氏弧菌与其鱿鱼宿主之间的共生开始期间，可以直接和真实的观察到，约5个费氏弧菌细胞在进入宿主组织之前沿着浅表上皮的粘膜纤毛膜聚集。在这里，我们表明，这些少数早期宿主相关的共生体特异性地诱导宿主基因表达的强烈变化，这对随后的定殖步骤至关重要。这种对其特定共生伙伴的极其敏感的反应包括宿主内切几丁质酶的上调，其活性将粘液中的聚合几丁质水解成壳二糖，从而引发共生体并产生促进费氏弧菌迁移到宿主组织中的化学引诱物梯度。因此，宿主在最初的共生体接触后转录响应，这有利于随后的殖民。
Upon transit to colonization sites, bacteria often experience critical priming that prepares them for subsequent, specific interactions with the host; however, the underlying mechanisms are poorly described. During initiation of the symbiosis between the bacterium Vibrio fischeri and its squid host, which can be observed directly and in real time, ~5 V. fischeri cells aggregate along the mucociliary membranes of a superficial epithelium prior to entering host tissues. Here we show that these few early host-associated symbionts specifically induce robust changes in host gene expression that are critical to subsequent colonization steps. This exquisitely sensitive response to its specific symbiotic partner includes the upregulation of a host endochitinase, whose activity hydrolyzes polymeric chitin in the mucus into chitobiose, thereby priming the symbiont and also producing a chemoattractant gradient that promotes V. fischeri migration into host tissues. Thus, the host responds transcriptionally upon initial symbiont contact, which facilitates subsequent colonization.
登录
查看更多内容
影响因子：
2
作者：
Heath-Heckman, Elizabeth A. C.;McFall-Ngai, Margaret J.
通讯作者：
McFall-Ngai, Margaret J.
影响因子：
56.9
作者：
Bry, L;Falk, PG;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
2.4
作者：
Hirano, T.;Aoki, M.;Nishio, T.
通讯作者：
Nishio, T.
影响因子：
4.4
作者：
Mandel, Mark J.;Schaefer, Amy L.;Ruby, Edward G.
通讯作者：
Ruby, Edward G.
影响因子：
3.1
作者：
Carlson, Steve A.;Sharma, Vijay K.;Franklin, Sharon K.
通讯作者：
Franklin, Sharon K.