The biological performance of cell-containing phospholipid polymer hydrogels in bulk and microscale form.
The biological performance of cell-containing phospholipid polymer hydrogels in bulk and microscale form.
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块状和微型形式的含细胞磷脂聚合物水凝胶的生物学性能。
DOI：
10.1016/j.biomaterials.2010.07.106
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发表时间：
2010
期刊：
影响因子：
14
通讯作者：
T. Kitamori
中科院分区：
文献类型：
--
作者：
Yan Xu;Kihoon Jang;T. Konno;K. Ishihara;K. Mawatari;T. Kitamori
研究并比较了块状和小型化形式的含细胞磷脂聚合物水凝胶的生物学性能，无需额外的培养基支持。当商业聚乙烯醇（PVA）和悬浮在细胞培养基中的细胞的磷脂聚合物聚[2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）-共-甲基丙烯酸正丁酯（BMA）-共-对乙烯基苯硼酸（VPBA）]（PMBV）溶液混合在一起时，自发形成含有细胞的水凝胶。分别在 96 孔微孔板和玻璃微芯片中制备厚度约为 3.1 mm 和 400 μm 的块状水凝胶和微型水凝胶。在这两种情况下，水凝胶都是均匀的，并且细胞在空间上被封装。细胞形态的长期观察（4天和8天）表明，细胞被动附着在水凝胶界面上，但无法扩散和压平，这抑制了两种水凝胶中的细胞生长。活力评估表明，两种水凝胶形式的细胞在长期封装后仍保持相同的高活力水平。细胞毒性测定表明，微型水凝胶中的细胞在细胞毒性敏感性方面与本体水凝胶和常规培养基培养物中的细胞保持高度相关。 PMBV/PVA水凝胶不仅为细胞长期存活提供了有益的细胞相容性微环境，无需额外的培养基支持，而且还为微芯片中的细胞维持创造了类似于散装细胞的静态条件。 PMBV/PVA 水凝胶在散装和小型化形式中具有一致的长期性能，使其成为开发长期、灵活、三维、基于活细胞的工具的理想选择，用于常规细胞分析和散装到微尺度水平的应用。
The biological performances of a cell-containing phospholipid polymer hydrogel in bulk and miniaturized formats without an additional culture medium support were investigated and compared. The cell-containing hydrogel was formed spontaneously when solutions of commercial polyvinyl alcohol (PVA) and the phospholipid polymer poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-vinylphenylboronic acid (VPBA)] (PMBV) suspended with cells in a cell culture medium are mixed together. Bulk and miniaturized hydrogels, with approximate thicknesses of 3.1 mm and 400 μm, respectively, were prepared in a 96-well microplate and a glass microchip, respectively. In both cases, the hydrogels were homogeneous, and cells were spatially encapsulated. The long-term observation (4 and 8 days) of cell morphology suggested that cells were passively attached to the interface of the hydrogel but were unable to spread and flatten, which inhibited cell growth in both hydrogels. Viability evaluations revealed that cells in both hydrogel formats maintained the same high viability levels after long-term encapsulation. Cytotoxicity assays indicated that the cells in the miniaturized hydrogel maintained a high degree of correlation in cytotoxic sensitivity with the cells in the bulk hydrogel and a routine medium culture. The PMBV/PVA hydrogel not only provides a beneficial cytocompatible microenvironment for long-term cell survival without an additional culture medium support but also creates a static condition for cell sustainment in a microchip similar to that in bulk. The uniform long-term performances of PMBV/PVA hydrogels in bulk and miniaturized formats make them ideal for the development of long-term, flexible, three-dimensional, living cell-based tools for routine cell-based assays and applications on bulk to microscale levels.
影响因子：
10.8
作者：
Siegel, Ronald A.;Gu, Yuandong;Lei, Ming;Baldi, Antonio;Nuxoll, Eric E.;Ziaie, Babak
通讯作者：
Ziaie, Babak
影响因子：
14
作者：
Kane, RS;Takayama, S;Whitesides, GM
通讯作者：
Whitesides, GM