Inhibitors of mitochondrial ATP production at the time of compaction improve development of in vitro produced porcine embryos
Inhibitors of mitochondrial ATP production at the time of compaction improve development of in vitro produced porcine embryos
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压缩时线粒体 ATP 生成的抑制剂可改善体外猪胚胎的发育
DOI：
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发表时间：
2001
影响因子：
2.5
通讯作者：
R. Prather
中科院分区：
文献类型：
--
作者：
Z. Machaty;J. Thompson;L. R. Abeydeera;B. N. Day;R. Prather
研究了紧实期线粒体ATP产生的部分抑制与猪胚胎发育的关系。在通过氧化磷酸化抑制ATP产生的条件下，体外生产的猪致密桑葚胚培养2天。培养条件包括：在培养基中添加氧化磷酸化抑制剂叠氮化钠（NaN3）；在2,4 -二硝基苯酚（DNP）存在下孵育，DNP是氧化磷酸化的解偶联剂；或在5% O2浓度下孵育。NaN3 （10 ~ 20 μM）能显著提高囊胚的平均核数（P < 0.05）。在100 μM DNP条件下发育的胚形成囊胚的发生率显著高于对照胚（P < 0.001）；这些囊胚的平均核数也较高（P < 0.005）。当这些处理从1细胞阶段开始应用时，它们被证明是有害的。在含5% O2和20% O2的气氛中培养致密的胚泡时，囊胚形成的频率（P < 0.05）和每个囊胚的平均核数（P < 0.001）也有所提高。NaN3或DNP对长期发育没有负面影响：处理过的胚胎在移植到受体后的第30天能够形成可存活的概念。这些数据表明，短暂抑制线粒体ATP的产生有利于猪胚胎的体外发育。摩尔。天线转换开关。开发。58:39 - 44,2001。©2001 Wiley‐Liss, Inc。
The relationship between partial inhibition of mitochondrial ATP production during the peri‐compaction stage and porcine embryonic development was studied. In vitro produced porcine compact morulae were cultured for two days under conditions that should inhibit ATP production via oxidative phosphorylation. The culture conditions included supplementation of the culture medium with sodium azide (NaN3), an oxidative phosphorylation inhibitor; incubation in the presence of 2,4‐dinitrophenol (DNP), an uncoupler of oxidative phosphorylation; or incubation under 5% O2 concentration. NaN3 (10–20 μM) increased the average nuclear number found in the resulting blastocysts (P < 0.05). The embryos developed in the presence of 100 μM DNP formed blastocysts at a significantly higher incidence than the control embryos (P < 0.001); the average nuclear number found in these blastocysts was also higher (P < 0.005). When these treatments were applied from the 1‐cell stage they proved to be detrimental. Elevations in the frequency of blastocyst formation (P < 0.05), and in the average nuclear number per blastocyst (P < 0.001) were also measured when compact morulae were incubated in an atmosphere containing 5% vs. 20% O2. NaN3 or DNP did not have negative effects on long term development: the treated embryos were able to form viable conceptuses by day 30 after being transferred into recipients. The data indicate that transient inhibition of mitochondrial ATP production is advantageous for porcine embryonic development in vitro. Mol. Reprod. Dev. 58:39–44, 2001. © 2001 Wiley‐Liss, Inc.
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在含 1% 至 20% 氧气的无蛋白培养基中将兔受精卵培养成囊胚。
DOI：
10.1530/jrf.0.0980163
发表时间：
1993
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Li,J;Foote,RH
通讯作者：
Foote,RH
影响因子：
3.6
作者：
Macháty, Z;Day, BN;Prather, RS
通讯作者：
Prather, RS
DOI：
10.1530/jrf.0.0990673
发表时间：
1993-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
FISCHER, B;BAVISTER, BD
通讯作者：
BAVISTER, BD
影响因子：
3.6
作者：
MCKIERNAN, SH;BAVISTER, BD
通讯作者：
BAVISTER, BD