Zeb1 regulation of wound-healing-induced inflammation in alkali-damaged corneas.
Zeb1 regulation of wound-healing-induced inflammation in alkali-damaged corneas.
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DOI：
10.1016/j.isci.2022.104038
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发表时间：
2022-04-15
期刊：
影响因子：
5.8
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Liang W;Zhang Y;Zhou L;Lu X;Finn ME;Wang W;Shao H;Dean DC;Zhang L;Liu Y
角膜是一种无血管组织，可保证视力清晰。碱烧伤可能会对角膜造成严重的创伤性损伤，并伴有炎症和新生血管形成 (NV)，导致视力下降和失明。角膜炎症和 NV 的机制尚不清楚。我们之前报道过 Zeb1 是角膜 NV 的重要因子，我们试图阐明它是否也参与角膜炎症的调节。我们通过多学科方法分析了 Zeb1+/+ 和 Zeb1−/+ 同窝仔鼠碱烧伤引起的角膜炎症和伤口愈合。我们提供的证据表明，Zeb1 与 Tgfb 形成正向调节环，通过维持免疫细胞活力和活动性来调节早期角膜炎症，并通过激活 Nf-κb 和 Tgfb 相关 Stat3 信号通路来调节后期伤口愈合。我们相信ZEB1是一个潜在的治疗靶点，ZEB1的失活可能是治疗严重角膜炎症的一种策略。创伤性伤口会诱发角膜炎症，导致视力下降 Zeb1 是保持免疫细胞活力、活动性和细胞因子表达的关键因素 Zeb1 通过 Nf-κb 和 Stat3 途径调节细胞因子基因表达 ZEB1 失活可能是治疗严重角膜炎症的一种策略生物科学；免疫学
The cornea is an avascular tissue for vision clarity. Alkali burn could cause severe traumatic damage on the cornea with inflammation and neovascularization (NV), leading to vision reduction and blindness. Mechanisms underlying corneal inflammation and NV are not as clear. We previously reported that Zeb1 is an important factor in corneal NV, and we sought to clarify whether it is also involved in regulation of corneal inflammation. We analyzed the alkali burn-induced corneal inflammation and wound healing in both Zeb1+/+ and Zeb1−/+ littermates through a multidisciplinary approach. We provide evidence that Zeb1 forms a positive regulatory loop with Tgfb to regulate early corneal inflammation by maintenance of immune cell viability and mobility and later wound healing by activation of both Nf-κb and Tgfb-related Stat3 signaling pathways. We believe that ZEB1 is a potential therapeutic target, and inactivation of ZEB1 could be a strategy to treat severe corneal inflammation condition. Traumatic wound induces inflammation in the cornea, resulting in vision reduction Zeb1 is a key factor to retain immune cell viability, mobility, and cytokine expression Zeb1 regulates cytokine gene expression through both Nf-κb and Stat3 pathways Inactivation of ZEB1 could be a strategy to treat severe corneal inflammation condition Ophthalmology; Biological sciences; Immunology
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影响因子：
39.3
作者：
Liu T;Zhang L;Joo D;Sun SC
通讯作者：
Sun SC
影响因子：
17.8
作者：
Ljubimov AV;Saghizadeh M
通讯作者：
Saghizadeh M
影响因子：
30.5
作者：
Sato, S;Sanjo, H;Akira, S
通讯作者：
Akira, S
DOI：
10.3791/51159
发表时间：
2014-04-07
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Anderson C;Zhou Q;Wang S
通讯作者：
Wang S
DOI：
10.1084/jem.185.8.1467
发表时间：
1997-04-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Higashi Y;Moribe H;Takagi T;Sekido R;Kawakami K;Kikutani H;Kondoh H
通讯作者：
Kondoh H