Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering.
Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering.
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DOI：
10.3390/bioengineering10070854
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发表时间：
2023-07-19
影响因子：
4.6
通讯作者：
Camci-Unal, Gulden
中科院分区：
文献类型：
--
作者：
Augustine, Robin;Nikolopoulos, Vasilios K.;Camci-Unal, Gulden
由创伤、疾病或老化引起的骨缺损在临床上提出了重大挑战。尽管骨组织工程的生物材料支架已经显示出有希望的结果，但挑战仍然存在，包括支架内需要足够的机械强度和合适的生物活性剂以促进骨形成。氧气是成功骨形成的关键因素，而低氧张力抑制了它。在本研究中，我们开发了明胶甲基丙烯酰（GelMA）水凝胶浸渍的静电纺丝聚己内酯（PCL）支架，可以释放氧气超过3周。我们研究了骨组织工程中细胞存活的复合支架的潜力。我们的研究结果表明，增加量的氧化钙纳米粒子的PCL支架显着增加氧气的产生，这是调制的GelMA浸渍。此外，所得到的支架显示出改善的细胞相容性，前成骨细胞粘附，和在缺氧条件下的增殖。这一发现是特别相关的，因为缺氧是一个普遍的特点，在各种骨疾病。除了提供氧气外，CaO2纳米颗粒还可作为增强剂，改善支架的机械性能，而GelMA的掺入可增强细胞粘附和增殖性能。总之，我们新开发的自氧化复合生物材料是骨组织工程应用的有前途的支架材料。
Bone defects resulting from trauma, disease, or aging present significant challenges in the clinic. Although biomaterial scaffolds for bone-tissue engineering have shown promising results, challenges remain, including the need for adequate mechanical strength and suitable bioactive agents within scaffolds to promote bone formation. Oxygen is a critical factor for successful bone formation, and low oxygen tension inhibits it. In this study, we developed gelatin methacryloyl (GelMA) hydrogel-impregnated electrospun polycaprolactone (PCL) scaffolds that can release oxygen over 3 weeks. We investigated the potential of composite scaffolds for cell survival in bone-tissue engineering. Our results showed that the addition of an increased amount of CaO2 nanoparticles to the PCL scaffolds significantly increased oxygen generation, which was modulated by GelMA impregnation. Moreover, the resulting scaffolds showed improved cytocompatibility, pre-osteoblast adhesion, and proliferation under hypoxic conditions. This finding is particularly relevant since hypoxia is a prevalent feature in various bone diseases. In addition to providing oxygen, CaO2 nanoparticles also act as reinforcing agents improving the mechanical property of the scaffolds, while the incorporation of GelMA enhances cell adhesion and proliferation properties. Overall, our newly developed self-oxygenating composite biomaterials are promising scaffolds for bone-tissue engineering applications.
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