Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis.
Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis.
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用姜黄素预处理脂肪干细胞通过抗凋亡和血管生成促进心肌恢复
DOI：
10.1155/2015/638153
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发表时间：
2015
影响因子：
4.3
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Liu J;Zhu P;Song P;Xiong W;Chen H;Peng W;Wang S;Li S;Fu Z;Wang Y;Wang H
干细胞移植在缺血心肌中的存活率低，限制了其治疗效果。姜黄素具有有效的抗氧化特性。这项研究调查了姜黄素治疗是否能保护干细胞免受氧化应激损伤，并改善细胞移植后的心肌恢复。用姜黄素或不用姜黄素预处理Autumn Sprague-Dawley大鼠脂肪来源的间充质干细胞（ADSC）。采用过氧化氢/血清剥夺（H2 O2/SD）培养液模拟离体缺血状态。评价姜黄素对ADSCs的细胞保护作用。姜黄素预处理可显著增加细胞活力和VEGF分泌，并通过调节PTEN/Akt/p53和HO-1信号蛋白的表达，减轻细胞损伤和凋亡。在心肌缺血再灌注损伤（IRI）模型中研究脂肪干细胞移植的治疗潜力。姜黄素预处理的脂肪干细胞移植不仅可改善心功能，提高细胞滞留率，缩小梗死面积，而且可减少心肌细胞凋亡，促进新生血管形成，增加缺血心肌VEGF水平。总之，用姜黄素引发ADSC改善了对氧化应激损伤的耐受性，并导致ADSC用于心肌修复的治疗潜力增强。姜黄素预处理是干细胞移植心肌修复的一种有前途的辅助策略。
The poor survival rate of transplanted stem cells in ischemic myocardium has limited their therapeutic efficacy. Curcumin has potent antioxidant property. This study investigates whether prior curcumin treatment protects stem cells from oxidative stress injury and improves myocardial recovery following cells transplantation. Autologous Sprague-Dawley rat adipose derived mesenchymal stem cells (ADSCs) were pretreated with or without curcumin. The hydrogen peroxide/serum deprivation (H2O2/SD) medium was used to mimic the ischemic condition in vitro. Cytoprotective effects of curcumin on ADSCs were evaluated. Curcumin pretreatment significantly increased cell viability and VEGF secretion, and decreased cell injury and apoptosis via regulation of PTEN/Akt/p53 and HO-1 signal proteins expression. The therapeutic potential of ADSCs implantation was investigated in myocardial ischemia-reperfusion injury (IRI) model. Transplantation of curcumin pretreated ADSCs not only resulted in better heart function, higher cells retention, and smaller infarct size, but also decreased myocardial apoptosis, promoted neovascularization, and increased VEGF level in ischemic myocardium. Together, priming of ADSCs with curcumin improved tolerance to oxidative stress injury and resulted in enhancement of their therapeutic potential of ADSCs for myocardial repair. Curcumin pretreatment is a promising adjuvant strategy for stem cells transplantation in myocardial restoration.
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影响因子：
--
作者：
Jiang, Bing-Hua;Liu, Ling-Zhi
通讯作者：
Liu, Ling-Zhi
Exendin-4干细胞佐剂通过STAT3激活修复心肌梗塞后的心脏
影响因子：
5.3
作者：
Liu J;Wang H;Wang Y;Yin Y;Du Z;Liu Z;Yang J;Hu S;Wang C;Chen Y
通讯作者：
Chen Y
影响因子：
3.9
作者：
Fleenor, Bradley S.;Sindler, Amy L.;Marvi, Natasha K.;Howell, Kate L.;Zigler, Melanie L.;Yoshizawa, Mutsuko;Seals, Douglas R.
通讯作者：
Seals, Douglas R.
影响因子：
3.3
作者：
Hodgetts, SI;Beilharz, MW;Grounds, MD
通讯作者：
Grounds, MD
DOI：
10.2152/jmi.54.177
发表时间：
2007-02-01
期刊：
The journal of medical investigation : JMI
影响因子：
--
作者：
Ieishi, Kiyoshi;Nomura, Masahiro;Ito, Susumu
通讯作者：
Ito, Susumu