Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta).
Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta).
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DOI：
10.1038/s41598-018-27271-x
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发表时间：
2018-06-20
影响因子：
4.6
通讯作者：
Kiyono T
中科院分区：
文献类型：
--
作者：
Fukuda T;Eitsuka T;Donai K;Kurita M;Saito T;Okamoto H;Kinoshita K;Katayama M;Nitto H;Uchida T;Onuma M;Sone H;Inoue-Murayama M;Kiyono T
保护濒危动物的遗传资源对后代至关重要。红海龟（Caretta caretta）是一种极度濒危物种，因为人类狩猎，与其他海龟物种杂交以及传染病。本研究建立了红海龟的原代成纤维细胞系，并对其种特异性染色体数目进行了分析，结果表明红海龟的种特异性染色体数目为2n = 56，与玳瑁和榄绿海龟的种特异性染色体数目一致。我们首先发现，由于相同的染色体数目，这使得多个海龟物种之间存在稳定的杂交，导致多个海龟物种之间的强烈杂交。与野生型细胞的细胞培养期相比，人源突变型细胞周期蛋白依赖性激酶4（CDK 4）和细胞周期蛋白D的表达显著延长了细胞培养期。重组成纤维细胞系保持了正常的染色体条件和形态，表明在G1/S期，控制细胞增殖的机制在各种脊椎动物中是进化保守的。据我们所知，这项研究是第一个证明功能保守，以克服负反馈系统，以限制哺乳动物和爬行动物之间的细胞周期的翻转。我们的细胞培养方法将能够共享来自极度濒危动物的细胞作为研究材料。
Conservation of the genetic resources of endangered animals is crucial for future generations. The loggerhead sea turtle (Caretta caretta) is a critically endangered species, because of human hunting, hybridisation with other sea turtle species, and infectious diseases. In the present study, we established primary fibroblast cell lines from the loggerhead sea turtle, and showed its species specific chromosome number is 2n = 56, which is identical to that of the hawksbill and olive ridley sea turtles. We first showed that intensive hybridization among multiple sea turtle species caused due to the identical chromosome number, which allows existence of stable hybridization among the multiple sea turtle species. Expressions of human-derived mutant Cyclin-dependent kinase 4 (CDK4) and Cyclin D dramatically extended the cell culture period, when it was compared with the cell culture period of wild type cells. The recombinant fibroblast cell lines maintained the normal chromosome condition and morphology, indicating that, at the G1/S phase, the machinery to control the cellular proliferation is evolutionally conserved among various vertebrates. To our knowledge, this study is the first to demonstrate the functional conservation to overcome the negative feedback system to limit the turn over of the cell cycle between mammalian and reptiles. Our cell culture method will enable the sharing of cells from critically endangered animals as research materials.
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影响因子：
3.7
作者：
Kuroda K;Kiyono T;Isogai E;Masuda M;Narita M;Okuno K;Koyanagi Y;Fukuda T
通讯作者：
Fukuda T
影响因子：
9.2
作者：
Jensen, Michael P.;Allen, Camryn D.;Dutton, Peter H.
通讯作者：
Dutton, Peter H.
影响因子：
3.7
作者：
HAYFLICK, L;MOORHEAD, PS
通讯作者：
MOORHEAD, PS
影响因子：
4.1
作者：
Donai, Kenichiro;Kiyono, Tohru;Fukuda, Tomokazu
通讯作者：
Fukuda, Tomokazu
影响因子：
3.1
作者：
KARL, SA;BOWEN, BW;AVISE, JC
通讯作者：
AVISE, JC