Single cell responses to spatially controlled photosensitized production of extracellular singlet oxygen.
Single cell responses to spatially controlled photosensitized production of extracellular singlet oxygen.
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DOI：
10.1111/j.1751-1097.2011.00951.x
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发表时间：
2011-09
影响因子：
3.3
通讯作者：
Ogilby PR
中科院分区：
文献类型：
--
作者：
Pedersen BW;Sinks LE;Breitenbach T;Schack NB;Vinogradov SA;Ogilby PR
检测了单个HeLa细胞对细胞外产生的单线态氧的反应。利用膜不透性的Pd-Pd-树状大分子作为光敏剂和聚焦激光的组合来控制单线态氧产生的空间域，聚焦激光用于局部敏化单线态氧的产生。接近单线态氧产生区域的细胞表现出通常与坏死性细胞死亡相关的形态变化。照射后在坏死前所经过的“等待期”取决于(A)细胞膜和照射区域之间的距离，(B)入射激光通量，以及由此产生的单线态氧的初始浓度，以及(C)单线态氧的寿命。这些数据表明，单线态氧在光诱导细胞死亡的过程中起着关键作用。使用细胞外产生的单线态氧诱导细胞死亡的方法可以解决一个经常限制细胞内光敏化细胞死亡的机制研究的问题：当使用细胞内光敏剂时，很难量化有效光剂量，从而量化单线态氧浓度。
The response of individual HeLa cells to extracellularly produced singlet oxygen was examined. The spatial domain of singlet oxygen production was controlled using the combination of a membrane-impermeable Pd porphyrin-dendrimer, which served as a photosensitizer, and a focused laser, which served to localize the sensitized production of singlet oxygen. Cells in close proximity to the domain of singlet oxygen production showed morphological changes commonly associated with necrotic cell death. The elapsed post-irradiation “waiting period” before necrosis became apparent depended on (a) the distance between the cell membrane and the domain irradiated, (b) the incident laser fluence and, as such, the initial concentration of singlet oxygen produced, and (c) the lifetime of singlet oxygen. The data imply that singlet oxygen plays a key role in this process of light-induced cell death. The approach of using extracellularly-generated singlet oxygen to induce cell death can provide a solution to a problem that often limits mechanistic studies of intracellularly photosensitized cell death: it can be difficult to quantify the effective light dose, and hence singlet oxygen concentration, when using an intracellular photosensitizer.
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影响因子：
3.1
作者：
Hatz, Sonja;Lambert, John D. C.;Ogilby, Peter R.
通讯作者：
Ogilby, Peter R.
影响因子：
3.2
作者：
DAHL, TA;MIDDEN, WR;HARTMAN, PE
通讯作者：
HARTMAN, PE
影响因子：
1.5
作者：
Kessel, D
通讯作者：
Kessel, D
影响因子：
3.3
作者：
DAHL, TA;MIDDEN, WR;HARTMAN, PE
通讯作者：
HARTMAN, PE
影响因子：
2.9
作者：
Dunphy, I;Vinogradov, SA;Wilson, DF
通讯作者：
Wilson, DF