Architecture of the native major royal jelly protein 1 oligomer.
Architecture of the native major royal jelly protein 1 oligomer.
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天然主要蜂王浆蛋白 1 寡聚物的结构
DOI：
10.1038/s41467-018-05619-1
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发表时间：
2018-08-22
影响因子：
16.6
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Tian W;Li M;Guo H;Peng W;Xue X;Hu Y;Liu Y;Zhao Y;Fang X;Wang K;Li X;Tong Y;Conlon MA;Wu W;Ren F;Chen Z
蜜蜂等级发育受皇家浆（RJ）的营养调节。蜂王浆主要蛋白1（Major Royal Jelly Protein 1，MRJP 1）是可溶皇家浆蛋白中含量最丰富的糖蛋白，在蜜蜂营养和幼虫发育中起着重要作用，并在人体内表现出广泛的药理活性。然而，它的结构长期以来一直是未知的。在此，我们确定并报告了一个16分子结构的天然MRJP 1寡聚体含有四个MRJP 1，四个apisimin，和八个未预料到的24-亚甲基胆固醇分子在2.65 μ m分辨率。MRJP 1具有独特的六叶β螺旋桨折叠结构，并含有三个二硫键，与芹菜素主要通过疏水作用相互作用。每四个24-亚甲基胆固醇分子被两个MRJP 1和两个apisimin分子包装。该组装体以保守和pH依赖的方式通过apisimin二聚化形成H形MRJP 14-apisimin 4 -24-亚甲基胆甾醇8复合物。我们的研究结果为理解MRJPs和24-亚甲基胆固醇的药理作用提供了结构基础，并提供了对它们在蜜蜂中独特生理作用的见解。
Honeybee caste development is nutritionally regulated by royal jelly (RJ). Major royal jelly protein 1 (MRJP1), the most abundant glycoprotein among soluble royal jelly proteins, plays pivotal roles in honeybee nutrition and larvae development, and exhibits broad pharmacological activities in humans. However, its structure has long remained unknown. Herein, we identify and report a 16-molecule architecture of native MRJP1 oligomer containing four MRJP1, four apisimin, and eight unanticipated 24-methylenecholesterol molecules at 2.65 Å resolution. MRJP1 has a unique six-bladed β-propeller fold with three disulfide bonds, and it interacts with apisimin mainly by hydrophobic interaction. Every four 24-methylenecholesterol molecules are packaged by two MRJP1 and two apisimin molecules. This assembly dimerizes to form an H-shaped MRJP14-apisimin4-24-methylenecholesterol8complex via apisimin in a conserved and pH-dependent fashion. Our findings offer a structural basis for understanding the pharmacological effects of MRJPs and 24-methylenecholesterol, and provide insights into their unique physiological roles in bees.
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DOI：
10.1155/2016/5040528
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Furusawa T;Arai Y;Kato K;Ichihara K
通讯作者：
Ichihara K
影响因子：
7
作者：
Chan QW;Chan MY;Logan M;Fang Y;Higo H;Foster LJ
通讯作者：
Foster LJ
影响因子：
3.5
作者：
Bíliková, K;Hanes, J;Simúth, J
通讯作者：
Simúth, J
影响因子：
4.4
作者：
Feng, Mao;Fang, Yu;Li, Jianke
通讯作者：
Li, Jianke
影响因子：
2.4
作者：
Hojo, Masaru;Kagami, Takahiro;Sasaki, Masami
通讯作者：
Sasaki, Masami