The Inhibitory Activity of the AU-Rich RNA Element in the Human Papillomavirus Type 1 Late 3′ Untranslated Region Correlates with Its Affinity for the elav-Like HuR Protein
The Inhibitory Activity of the AU-Rich RNA Element in the Human Papillomavirus Type 1 Late 3′ Untranslated Region Correlates with Its Affinity for the elav-Like HuR Protein
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人乳头瘤病毒 1 型晚期 3 非翻译区中富含 AU 的 RNA 元件的抑制活性与其对 elav 样 HuR 蛋白的亲和力相关
DOI：
10.1128/jvi.73.2.1080-1091.1999
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发表时间：
1999
影响因子：
5.4
通讯作者：
S. Schwartz
中科院分区：
文献类型：
--
作者：
M. Sokolowski;H. Furneaux;S. Schwartz
摘要人乳头瘤病毒1型晚期3′非翻译区中一个57个核苷酸的富含腺苷和尿苷的RNA不稳定元件medh 1ARE先前已被证明与三种不能与失活突变RNA结合的核蛋白特异性相互作用。其中两个被鉴定为异质性核糖核蛋白C1和C2，而第三个，一个38 kDa的多聚（U）结合蛋白（p38），仍然没有被鉴定。在这里，我们表明，部分纯化的p38与最近确定的elav样HuR蛋白提出的单克隆抗体反应，表明p38是HuR蛋白。事实上，重组谷胱甘肽S-转移酶（GST）-HuR蛋白特异性结合h1 ARE内的位点。对h1 ARE及其失活突变体的GST-HuR的表观Kd值的测定显示，GST-HuR与野生型序列的结合亲和力高出50倍以上。因此，GST-HuR对野生型和mutanth 1ARE的结合亲和力与它们在转染细胞中的抑制活性相关，强烈表明HuR蛋白参与人乳头瘤病毒1型晚期基因表达的转录后调节。
ABSTRACT A 57-nucleotide adenosine- and uridine-rich RNA instability element in the human papillomavirus type 1 late 3′ untranslated region termedh1ARE has previously been shown to interact specifically with three nuclear proteins that failed to bind to an inactive mutant RNA. Two of those were identified as the heterogeneous ribonucleoproteins C1 and C2, whereas the third, a 38-kDa, poly(U) binding protein (p38), remained unidentified. Here we show that partially purified p38 reacts with a monoclonal antibody raised against the recently identified elav-like HuR protein, indicating that p38 is the HuR protein. Indeed, recombinant glutathioneS-transferase (GST)-HuR protein binds specifically to sites within the h1ARE. Determination of the apparentKd value of GST-HuR for the h1ARE and the inactive mutant thereof revealed that GST-HuR bound with a more than 50-fold-higher affinity to the wild-type sequence. Therefore, the binding affinity of GST-HuR for the wild-type and mutanth1AREs correlates with their inhibitory activities in transfected cells, strongly suggesting that the HuR protein is involved in the posttranscriptional regulation of human papillomavirus type 1 late-gene expression.
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影响因子：
9.3
作者：
Moat, Stuart J.;Rees, Derek;Hillier, Sharon
通讯作者：
Hillier, Sharon
一种人类 RNase E 样活性，可切割参与 mRNA 稳定性控制的 RNA 序列。
DOI：
10.1073/pnas.92.16.7322
发表时间：
1995
影响因子：
11.1
作者：
Wennborg,A;Sohlberg,B;Angerer,D;Klein,G;vonGabain,A
通讯作者：
vonGabain,A
影响因子：
1.9
作者：
CAMPOS, AR;GROSSMAN, D;WHITE, K
通讯作者：
WHITE, K
DOI：
10.1073/pnas.91.23.11207
发表时间：
1994-11-08
影响因子：
11.1
作者：
GAO, FB;CARSON, CC;KEENE, JD
通讯作者：
KEENE, JD