Diversity and dynamics of the Drosophila transcriptome.
Diversity and dynamics of the Drosophila transcriptome.
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动物的转录本是动态的，每种细胞类型、组织和器官系统都表达一系列转录异构体，从而产生实质性的多样性。我们使用Poly(A)+RNA序列从果蝇培养的黑腹果蝇细胞系、解剖的器官系统和环境扰动中鉴定出新的基因、转录本和蛋白质。我们发现，一小部分主要是神经特异的基因有可能通过广泛的替代启动子使用和RNA剪接来编码数千个转录本。组织之间的剪接变化幅度大于发育阶段之间的剪接变化，并且大多数性别特异的剪接是性腺特异的。性腺表达数百个以前未知的编码和长非编码RNA(LncRNAs)，其中一些与蛋白质编码基因反义，并产生短调控RNA。此外，以前发现的普遍基因间转录主要发生在新发现的内含子中。苍蝇转录组比以前认识的复杂得多，是由启动子、剪接位点和多聚腺苷酸化位点的组合使用引起的。
Animal transcriptomes are dynamic, each cell type, tissue and organ system expressing an ensemble of transcript isoforms that give rise to substantial diversity. We identified new genes, transcripts, and proteins using poly(A)+ RNA sequence from Drosophila melanogaster cultured cell lines, dissected organ systems, and environmental perturbations. We found a small set of mostly neural-specific genes has the potential to encode thousands of transcripts each through extensive alternative promoter usage and RNA splicing. The magnitudes of splicing changes are larger between tissues than between developmental stages, and most sex-specific splicing is gonad-specific. Gonads express hundreds of previously unknown coding and long noncoding RNAs (lncRNAs) some of which are antisense to protein-coding genes and produce short regulatory RNAs. Furthermore, previously identified pervasive intergenic transcription occurs primarily within newly identified introns. The fly transcriptome is substantially more complex than previously recognized arising from combinatorial usage of promoters, splice sites, and polyadenylation sites.
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影响因子：
48
作者：
Katz, Yarden;Wang, Eric T.;Airoldi, Edoardo M.;Burge, Christopher B.
通讯作者：
Burge, Christopher B.
影响因子：
46.9
作者：
Boley N;Stoiber MH;Booth BW;Wan KH;Hoskins RA;Bickel PJ;Celniker SE;Brown JB
通讯作者：
Brown JB
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
人类和小鼠基因组中的复杂基因座。
影响因子：
4.5
作者：
Engstrom, Par G.;Suzuki, Harukazu;Ninomiya, Noriko;Akalin, Altuna;Sessa, Luca;Lavorgna, Giovanni;Brozzi, Alessandro;Luzi, Lucilla;Tan, Sin Lam;Yang, Liang;Kunarso, Galih;ng, Edwin Lian-Cho Ng;Batalov, Serge;Wahlestedt, Claes;Kai, Chikatoshi;Kawai, Jun;Carninci, Piero;Hayashizaki, Yoshihide;Wells, Christine;Bajic, Vladimir B.;Orlando, Valerio;Reid, James F.;Lenhard, Boris;Lipovich, Leonard
通讯作者：
Lipovich, Leonard
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE