Genetic determinants of macrolide and tetracycline resistance in penicillin non-susceptible Streptococcus pneumoniae isolates from people living with HIV in Dar es Salaam, Tanzania.
Genetic determinants of macrolide and tetracycline resistance in penicillin non-susceptible Streptococcus pneumoniae isolates from people living with HIV in Dar es Salaam, Tanzania.
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DOI：
10.1186/s12941-023-00565-3
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发表时间：
2023-02-20
影响因子：
5.7
通讯作者：
Blomberg, Bjorn
中科院分区：
文献类型：
--
作者：
Manyahi, Joel;Moyo, Sabrina J. J.;Langeland, Nina;Blomberg, Bjorn
每年有100多万人死于肺炎链球菌，艾滋病毒感染者尤其脆弱。新出现的青霉素不敏感肺炎链球菌（PNSP）挑战肺炎球菌疾病的治疗。本研究的目的是通过下一代测序确定PNSP分离株的抗生素耐药机制。我们评估了从坦桑尼亚达累斯萨拉姆的537名健康人类免疫缺陷病毒（HIV）感染成人的鼻咽中获得的26株PNSP分离株，这些成人参加了随机临床试验CoTrimResist（ClinicalTrials.gov标识符：NCT 03087890，于2017年3月23日注册）。使用Illumina平台上的下一代全基因组测序来鉴定PNSP中对抗生素的抗性机制。50%（13/26）的PNSP对红霉素耐药，其中MLSB表型占54%（7/13），M表型占46%（6/13）。所有红霉素耐药PNSP均携带大环内酯类耐药基因; 6株具有mef（A）-msr（D），5株同时具有mef（B）和mef（A）-msr（D），2株仅携带mef（B）。与不含β-内酰胺酶（B）基因的分离株（MIC 4 - 12 μ g/mL）相比，携带β-内酰胺酶（B）基因的分离株对大环内酯类的MIC（> 256 μ g/mL）增加，p <0.001。使用欧洲抗菌药物敏感性试验委员会（EUCAST）指南，与遗传相关性相比，阿奇霉素耐药的患病率被高估。26株PNSP中有13株（50%）对四环素耐药，且均携带tet（M）基因。所有携带tet（M）基因的菌株和11/13携带大环内酯类耐药基因的菌株与移动的遗传元件Tn6009转座子家族相关。26株PNSP中，血清型3最常见（6/26），序列型ST 271占15%（4/26）。血清型3和19显示高水平的大环内酯类耐药，并经常携带大环内酯类和四环素耐药基因。其中，mesr（B）和mef（A）-msr（D）是PNSP对MLSB的共同抗性基因。四环素抗性由tet（M）基因赋予。抗性基因与Tn6009转座子相关。
Over one million yearly deaths are attributable to Streptococcus pneumoniae and people living with HIV are particularly vulnerable. Emerging penicillin non-susceptible Streptococcus pneumoniae (PNSP) challenges therapy of pneumococcal disease. The aim of this study was to determine the mechanisms of antibiotic resistance among PNSP isolates by next generation sequencing. We assessed 26 PNSP isolates obtained from the nasopharynx from 537 healthy human immunodeficiency virus (HIV) infected adults in Dar es Salaam, Tanzania, participating in the randomized clinical trial CoTrimResist (ClinicalTrials.gov identifier: NCT03087890, registered on 23rd March, 2017). Next generation whole genome sequencing on the Illumina platform was used to identify mechanisms of resistance to antibiotics among PNSP. Fifty percent (13/26) of PNSP were resistant to erythromycin, of these 54% (7/13) and 46% (6/13) had MLSB phenotype and M phenotype respectively. All erythromycin resistant PNSP carried macrolide resistance genes; six isolates had mef(A)-msr(D), five isolates had both erm(B) and mef(A)-msr(D) while two isolates carried erm(B) alone. Isolates harboring the erm(B) gene had increased MIC (> 256 µg/mL) towards macrolides, compared to isolates without erm(B) gene (MIC 4-12 µg/mL) p < 0.001. Using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines, the prevalence of azithromycin resistance was overestimated compared to genetic correlates. Tetracycline resistance was detected in 13/26 (50%) of PNSP and all the 13 isolates harbored the tet(M) gene. All isolates carrying the tet(M) gene and 11/13 isolates with macrolide resistance genes were associated with the mobile genetic element Tn6009 transposon family. Of 26 PNSP isolates, serotype 3 was the most common (6/26), and sequence type ST271 accounted for 15% (4/26). Serotypes 3 and 19 displayed high-level macrolide resistance and frequently carried both macrolide and tetracycline resistance genes. The erm(B) and mef(A)-msr(D) were common genes conferring resistance to MLSB in PNSP. Resistance to tetracycline was conferred by the tet(M) gene. Resistance genes were associated with the Tn6009 transposon.
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DOI：
10.1016/j.ijantimicag.2018.04.015
发表时间：
2018-12-01
影响因子：
10.8
作者：
Raddaoui, Anis;Ben Tanfous, Farah;Benhassen, Assia
通讯作者：
Benhassen, Assia
影响因子：
3.2
作者：
Diawara, Idrissa;Zerouali, Khalid;Elmdaghri, Naima
通讯作者：
Elmdaghri, Naima
DOI：
10.1016/s2214-109x(18)30247-x
发表时间：
2018-07
期刊：
The Lancet. Global health
影响因子：
--
作者：
Wahl B;O'Brien KL;Greenbaum A;Majumder A;Liu L;Chu Y;Lukšić I;Nair H;McAllister DA;Campbell H;Rudan I;Black R;Knoll MD
通讯作者：
Knoll MD
影响因子：
11.8
作者：
Mudhune, Sandra;Wamae, Maranga
通讯作者：
Wamae, Maranga
DOI：
10.1080/2159256x.2017.1368433
发表时间：
2017-01-01
期刊：
Mobile genetic elements
影响因子：
--
作者：
Sansevere, Emily A;Robinson, D Ashley
通讯作者：
Robinson, D Ashley