Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism.
Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism.
复制标题
枯草芽孢杆菌对丝氨酸的抗性：丝氨酸转运蛋白YbeC和连接丝氨酸和苏氨酸代谢的代谢网络的鉴定。
DOI：
10.1111/1462-2920.15179
复制
发表时间：
2020-09
影响因子：
5.1
通讯作者：
Stülke J
中科院分区：
文献类型：
--
作者：
Klewing A;Koo BM;Krüger L;Poehlein A;Reuß D;Daniel R;Gross CA;Stülke J
革兰阳性细菌枯草芽孢杆菌不仅使用丝氨酸作为蛋白质的构建块，而且在许多合成代谢反应中也是重要的前体。此外，缺乏丝氨酸会导致生物膜形成的启动。然而，过量的丝氨酸抑制枯草芽孢杆菌的生长。为了揭示潜在的机制，我们通过三种靶向和非靶向全基因组筛选分离出能够耐受有毒丝氨酸浓度的抑制突变体。所有筛选以及在大肠杆菌中的遗传互补均鉴定出迄今尚未鉴定的渗透酶YbeC是枯草芽孢杆菌的主要丝氨酸转运体。除YbeC外，苏氨酸转运蛋白BcaP和YbxG对丝氨酸摄取也有少量贡献。缺乏这三种转运体的菌株能够耐受100 mM丝氨酸，而野生型菌株已经被1 mM的氨基酸抑制。丝氨酸抗性突变体的筛选还发现了导致丝氨酸降解增加和苏氨酸生物合成酶表达增加的突变，这表明丝氨酸毒性是由干扰苏氨酸生物合成引起的。
The Gram‐positive bacterium Bacillus subtilis uses serine not only as a building block for proteins but also as an important precursor in many anabolic reactions. Moreover, a lack of serine results in the initiation of biofilm formation. However, excess serine inhibits the growth of B. subtilis. To unravel the underlying mechanisms, we isolated suppressor mutants that can tolerate toxic serine concentrations by three targeted and non‐targeted genome‐wide screens. All screens as well as genetic complementation in Escherichia coli identified the so far uncharacterized permease YbeC as the major serine transporter of B. subtilis. In addition to YbeC, the threonine transporters BcaP and YbxG make minor contributions to serine uptake. A strain lacking these three transporters was able to tolerate 100 mM serine whereas the wild type strain was already inhibited by 1 mM of the amino acid. The screen for serine‐resistant mutants also identified mutations that result in increased serine degradation and in increased expression of threonine biosynthetic enzymes suggesting that serine toxicity results from interference with threonine biosynthesis.
登录
查看更多内容
影响因子：
3.2
作者：
Belitsky, Boris R.
通讯作者：
Belitsky, Boris R.
影响因子：
3.2
作者：
CALOGERO, S;GARDAN, R;DEBARBOUILLE, M
通讯作者：
DEBARBOUILLE, M
影响因子：
7
作者：
Muntel, Jan;Fromion, Vincent;Becher, Doerte
通讯作者：
Becher, Doerte
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1093/bioinformatics/bts199
发表时间：
2012-06-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Kearse M;Moir R;Wilson A;Stones-Havas S;Cheung M;Sturrock S;Buxton S;Cooper A;Markowitz S;Duran C;Thierer T;Ashton B;Meintjes P;Drummond A
通讯作者：
Drummond A