Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2-apatite composite layer coating.
Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2-apatite composite layer coating.
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DOI：
10.1186/s13018-016-0501-z
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发表时间：
2017-01-05
影响因子：
2.6
通讯作者：
Yamazaki M
中科院分区：
文献类型：
--
作者：
Fujii K;Ito A;Mutsuzaki H;Murai S;Sogo Y;Hara Y;Yamazaki M
螺钉松动是骨科颌面外科常见的问题。已经尝试并报告了修改植入物表面以提高螺钉的机械强度。我们开发了涂覆有成纤维细胞生长因子-2（FGF-2）-磷灰石复合层的螺钉。然后，我们在经皮外固定模型中表明，该复合层具有缓慢保持和释放FGF-2的能力，从而降低了经皮外固定的针道感染的风险。本研究的目的是阐明钛螺钉上FGF-2−磷灰石复合层对螺钉周围骨形成的影响。我们分析了先前进行的动物实验的样品。通过将螺钉浸入含有FGF-2的过饱和磷酸钙溶液中，使螺钉涂覆FGF-2−磷灰石复合层。然后，将无涂层、磷灰石涂层和FGF-2−磷灰石复合层涂层螺钉经皮植入家兔体内。最后，使用无炎症的组织切片，我们组织形态计量学评估它们是否存在骨形成。然后对数据进行威布尔图分析。平均而言，FGF-2−磷灰石复合涂层螺钉的骨沉积率显著高于未涂层或磷灰石涂层螺钉。尽管平均骨沉积率的差异很小，但与没有FGF-2（无涂层和磷灰石涂层螺钉）的发生率相比，FGF-2−磷灰石复合材料层显著降低了螺钉周围骨形成受损的发生率。例如，骨沉积率等于或小于63.75%的概率对于涂覆有FGF-2−磷灰石复合层的螺钉为3.5 × 10-4，而对于没有FGF-2的螺钉为0.05。FGF-2-磷灰石复合涂层显著降低了骨与螺钉附着受损的风险。因此，使用带有FGF-2−磷灰石复合层涂层的钛螺钉作为内固定螺钉来降低松动风险是可行的。
Loosening of screws is a common problem in orthopedic and maxillofacial surgery. Modifying the implant surface to improve the mechanical strength of screws has been tried and reported. We developed screws coated with fibroblast growth factor-2 (FGF-2)−apatite composite layers. We then showed, in a percutaneous external fixation model, that this composite layer had the ability to hold and release FGF-2 slowly, thereby reducing the risk of pin tract infection of the percutaneous external fixation. The objective of the current study was to clarify the effect of FGF-2−apatite composite layers on titanium screws on bone formation around the screw. We analyzed samples of previously performed animal experiments. The screws were coated with FGF-2−apatite composite layers by immersing them in supersaturated calcium phosphate solutions containing FGF-2. Then, the uncoated, apatite-coated, and FGF-2−apatite composite layer-coated screws were implanted percutaneously in rabbits. Finally, using inflammation-free histological sections, we histomorphometrically assessed them for the presence of bone formation. Weibull plot analysis was then applied to the data. On average, screws coated with FGF-2−apatite composite layers showed a significantly higher bone apposition rate than the uncoated or apatite-coated screws. Although the difference in the average bone apposition rate was small, the FGF-2−apatite composite layers produced a significant, marked reduction in the incidence of impaired bone formation around the screw compared with the incidence in the absence of FGF-2 (uncoated and apatite-coated screws). The probability of resulting in a bone apposition rate equal to or less than 63.75%, for example, is 3.5 × 10-4 for screws coated with the FGF-2−apatite composite layers versus 0.05 for screws in the absence of FGF-2. FGF-2-apatite composite layer coating significantly reduced the risk of impaired bone apposition to the screw. Thus, it is feasible to use titanium screws coated with FGF-2−apatite composite layers as internal fixation screws to decrease the risk of loosening.
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影响因子：
5.6
作者：
Mutsuzaki H;Ito A;Sogo Y;Sakane M;Oyane A;Yamazaki M
通讯作者：
Yamazaki M
影响因子：
4
作者：
Sogo, Yu;Ito, Atsuo;Ichinose, Noboru
通讯作者：
Ichinose, Noboru
影响因子：
2.8
作者：
Inoue, Gen;Ueno, Masaki;Takaso, Masashi
通讯作者：
Takaso, Masashi
影响因子：
4.1
作者：
Gao, Ying;Luo, En;Li, Jihua
通讯作者：
Li, Jihua
影响因子：
3
作者：
HALVORSON, TL;KELLEY, LA;COOK, SD
通讯作者：
COOK, SD