The calcium phosphate matrix of FGF-2-apatite composite layers contributes to their biological effects.
The calcium phosphate matrix of FGF-2-apatite composite layers contributes to their biological effects.
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DOI：
10.3390/ijms150610252
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发表时间：
2014-06-10
影响因子：
5.6
通讯作者：
Yamazaki M
中科院分区：
文献类型：
--
作者：
Mutsuzaki H;Ito A;Sogo Y;Sakane M;Oyane A;Yamazaki M
本研究的目的是在25 °C下使用过饱和磷酸钙（CaP）溶液在钛（Ti）针上一步制备成纤维细胞生长因子（FGF）-2-磷灰石复合层，并与在37 °C下制备的涂层钛针相比，评价涂层钛针的理化特性和生物学效应。将钛针浸入含0.5、1.0或2.0 µg/mL FGF-2的过饱和CaP溶液中，在25 ℃下持续24 h（25F0.5、25F1.0和25F2.0），或在含4.0 µg/mL FGF-2的过饱和CaP溶液中，在37 ℃下持续48 h（37F4.0）。除了25F0.5之外，通过这两种方法形成的复合层的化学组成和FGF-2的促有丝分裂活性水平是相似的，除了Ca/P摩尔比，其在25 °C下（1.55-1.56 ± 0.01-0.02，p = 0.0008-0.0045）显著小于在37 °C下（1.67 ± 0.11）。因此，在25 °C下形成的复合层中，磷灰石不太成熟或者无定形磷酸钙的量较高。在体内，37 F4.0（45%）目视检查的针道感染率低于25 F1.0（80%，p = 0.0213），37 F4.0（35%）的骨髓炎率低于25 F0.5（75%，p = 0.0341）。37 F4.0的拔出扭矩（0.276 ± 0.117 Nm）高于25 F0.5（0.192 ± 0.117 Nm，p = 0.0142）和25 F1.0（0.176 ± 0.133 Nm，p = 0.0079）。S. 37F4.0的金黄色葡萄球菌（35%）低于25F0.5（75%，p = 0.0110）。总的来说，在25 °C下形成的FGF-2-磷灰石复合层在改善骨-针界面的固定强度和抵抗针道感染方面往往不太有效。这些结果表明，除了FGF-2含量和活性之外，包埋FGF-2的磷酸钙基质的化学性质对复合材料的抗感染性和固定强度具有显著影响。
The purpose of the present study was to fabricate fibroblast growth factor (FGF)-2-apatite composite layers on titanium (Ti) pins in one step at 25 °C using a supersaturated calcium phosphate (CaP) solution, and to evaluate the physicochemical characteristics and biological effects of the coated Ti pins compared with coated Ti pins fabricated at 37 °C. Ti pins were immersed in a supersaturated CaP solution containing 0.5, 1.0, or 2.0 µg/mL FGF-2 at 25 °C for 24 h (25F0.5, 25F1.0, and 25F2.0) or containing 4.0 µg/mL FGF-2 at 37 °C for 48 h (37F4.0). Except for the 25F0.5, the chemical compositions and the mitogenic activity levels of FGF-2 of the composite layers formed by these two methods were similar, except for the Ca/P molar ratio, which was markedly smaller at 25 °C (1.55–1.56 ± 0.01–0.02, p = 0.0008–0.0045) than at 37 °C (1.67 ± 0.11). Thus, either the apatite was less mature or the amount of amorphous calcium phosphate was higher in the composite layer formed at 25 °C. In vivo, the pin tract infection rate by visual inspection for 37F4.0 (45%) was lower than that for 25F1.0 (80%, p = 0.0213), and the rate of osteomyelitis for 37F4.0 (35%) was lower than that for 25F0.5 (75%, p = 0.0341). The extraction torque for 37F4.0 (0.276 ± 0.117 Nm) was higher than that for 25F0.5 (0.192 ± 0.117 Nm, p = 0.0142) and that for 25F1.0 (0.176 ± 0.133 Nm, p = 0.0079). The invasion rate of S. aureus for 37F4.0 (35%) was lower than that for 25F0.5 (75%, p = 0.0110). On the whole, the FGF-2-apatite composite layer formed at 25 °C tended to be less effective at improving fixation strength in the bone-pin interface and resisting pin tract infections. These results suggest that the chemistry of the calcium phosphate matrix that embeds FGF-2, in addition to FGF-2 content and activity, has a significant impact on composite infection resistance and fixation strength.
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