Identification of environmental chemicals that induce yolk malabsorption in zebrafish using automated image segmentation.
Identification of environmental chemicals that induce yolk malabsorption in zebrafish using automated image segmentation.
复制标题
DOI：
10.1016/j.reprotox.2014.10.022
复制
发表时间：
2015-08-01
影响因子：
3.3
通讯作者：
Bondesson, Maria
中科院分区：
文献类型：
--
作者：
Kalasekar, Sharanya Maanasi;Zacharia, Eleni;Kessler, Noah;Ducharme, Nicole A.;Gustafsson, Jan-Ake;Kakadiaris, Ioannis A.;Bondesson, Maria
影响发育过程中营养可用性的环境因素可能会产生长期影响，包括在以后的生活中易患疾病。为了确定环境中能够改变营养动员的化学物质，我们分析了斑马鱼胚胎中的卵黄吸收不良，斑马鱼胚胎在生命的第一周依赖母体卵黄获取营养。转基因斑马鱼品系 HGn50D 的胚胎在斑马鱼的卵黄合胞体层、心脏和眼睛中发出荧光，受精后两到五天将其胚胎暴露于九种化学物质中，包括农药（咪鲜胺、抑霉唑和丁乐林）、药品（氯贝丁酯和吉非罗齐）、阻燃剂（四溴双酚 A 和四氯双酚） A)、表面活性剂（全氟辛酸）和杀菌剂（三丁基锡）。我们开发了一个名为 ZebRA 的软件包，可以自动准确地分割和量化治疗期结束时捕获的图像中荧光蛋黄的面积。基于此定量，我们发现咪鲜胺降低了蛋黄吸收，而丁乐灵、四溴双酚 A、四氯双酚 A 和三丁基锡在测试的较高浓度下增加了蛋黄吸收。鉴于当今商业中工业化学品的数量和种类，开发自动化图像处理以实现高速分析和准确量化生物效应是实现高通量筛选以识别对营养吸收产生不利影响的化学品的重要一步。
Environmental factors affecting nutrient availability during development can have long-term effects including predisposition to diseases later in life. In order to identify chemicals in the environment capable of altering nutrient mobilization, we analyzed yolk malabsorption in the zebrafish embryo, which relies on maternally-derived yolk for nutrition during the first week of life. Embryos of the transgenic zebrafish line HGn50D, which fluoresce in the yolk syncytial layer, the heart, and the eyes of the zebrafish, were exposed from two to five days post fertilization to nine chemicals, including pesticides (prochloraz, imazalil, and butralin), pharmaceuticals (clofibrate and gemfibrozil), flame retardants (tetrabromobisphenol A, and tetrachlorobisphenol A), a surfactant (perfluorooctanoic acid) and a biocide (tributyltin). We developed a software package named ZebRA to automatically and accurately segment and quantify the area of the fluorescing yolk in images captured at the end of the treatment period. Based on this quantification, we found that prochloraz decreased yolk absorption, while butralin, tetrabromobisphenol A, tetrachlorobisphenol A and tributyltin, at the higher concentrations tested, increased yolk absorption. Given the number and variety of industrial chemicals in commerce today, development of automated image processing allowing for high-speed analysis and accurate quantification of biological effects is an important step for enabling high throughput screening to identify chemicals adversely impacting nutrient absorption.
登录
查看更多内容
DOI：
10.3791/4196
发表时间：
2012-11-18
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Avdesh A;Chen M;Martin-Iverson MT;Mondal A;Ong D;Rainey-Smith S;Taddei K;Lardelli M;Groth DM;Verdile G;Martins RN
通讯作者：
Martins RN
DOI：
10.1001/jama.2014.732
发表时间：
2014-02-26
期刊：
JAMA
影响因子：
--
作者：
Ogden CL;Carroll MD;Kit BK;Flegal KM
通讯作者：
Flegal KM
影响因子：
3.5
作者：
Inadera, H;Shimomura, A
通讯作者：
Shimomura, A
影响因子：
1.8
作者：
Muto, N;Hirai, H;Tanaka, K
通讯作者：
Tanaka, K
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF